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ResponsibilitiesExtract protein, DNA and/or RNA from necropsied animal tissueRun ddPCR, qPCR, ELISA, Western Blot etc analysis on tissue extractsCell culture, Neutralizing antibody assays and total antibody assaysSample analysis assay optimizationSOP writingData recording and analysisQC analysis This position directly impacts preclinical outcomes including proof of concept, ED50, MED, clinical hold response and IND enabling studies.
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Assists with various cell culture techniques such as performing flow cytometry analysis of cell surface protein expression, cell propagation, contaminant testing, and cryopreservation. Ability to perform light, fluorescence and confocal microscopy techniques to visualize protein expression in tissue sections.
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Hands-on expertise in tissue culture and cell-based assays (including general tissue culture, cell banking, cell proliferation, electroporation, transfection, transduction, flow cytometry, AAV, and ELISA.
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Experience with routine monitoring via tissue-culture microscopy. Inventory management and maintenance of tissue culture laboratory reagents. The Associate Scientist/Scientist I will be given the opportunity to develop broader experience in advanced cell culture techniques, diverse automation platforms and emerging technology.
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Experience with mammalian tissue culture, aseptic technique, flow cytometry, western blot, Simple Western, MSD, ELISA. Fit with Nurix Culture and Values. Experience with mammalian tissue culture, aseptic technique, flow cytometry, western blot, Simple Western, MSD, ELISA.
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Expertise in mammalian cell culture using aseptic tissue culture techniques and cell-based assays to evaluate viability (siRNA/shRNA, small molecule compound screening), protein level analysis (western blot), gene expression analysis (qRT-PCR) and other functional/molecular biology assays.
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Lab bench skills including pipetting, tissue culture, antibody staining. We are committed to building a broadly diverse community, nurturing a culture that is welcoming and supportive, and engaging diverse ideas for the provision of culturally competent education, discovery, and patient care.
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Familiarity with cell/tissue culture, cell-based assays, and microscopy platforms; working knowledge of modern molecular and cell biology techniques. Leveraging our proprietary opto-electropositioning (OEP) technology to move particles, the Beacon system enables single-cell isolation, culture, and a multitude of assays.
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Hands-on experience in tissue culture and cell-based assay (general tissue culture, cell banking, cell proliferation, electroporation, transfection, transduction, flow cytometry, AAV, and ELISA.
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Candidate must have strong technical skills in mammalian cell culture, and cell-based assay development using cell lines and primary cells. Working with Human/primate tissue and blood is nice to have.
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Candidate must have diverse laboratory experience which includes, but is not limited to, molecular biology, flow cytometry, and/or immunological-based assays (e.g. MSD, ELISA, WB). BS degree with 5+ years of relevant experience; or MS degree with 3+ years or PHD with 1 year of relevant experience within laboratory and industry, preferably in Cancer Biology, Cell Biology, Genetics, Pharmacology, Immunology, or a related scientific discipline.
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Knowledge of and experience with basic cell and molecular biology protocols: tissue culture, PCR, DNA sequence analysis and cloning; comfort working with instrumentation and computers; maintenance of accurate notebooks and computer files; team player with good communication and interpersonal skills; willingness to learn, seek out advice and admit errors; highly motivated to succeed.
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Perform molecular biology procedures, including DNA and RNA isolation, PCR, qRT-PCR, cloning, tissue culture, western blot analysis, and possibly others. Experience in cloning, tissue culture, patient sample handling, western blot analysis, PCR, qRT-PCR.
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We use a broad range of techniques from high resolution imaging, bioinformatics, cell biology and biochemical assays, viral-mediated gene delivery, functional and molecular studies in vivo and in vitro, using tissue culture and mouse models.
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As our Research Associate - Tissue Culture and Transformation, you will play an essential role in our tissue culture and transformation production systems in woody species.
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tissue culture jobs in San Francisco, CA
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