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Extensive hands-on experience with Molecular Biology (as PCR, qRT-PCR, ddPCR, DNA/RNA and protein preparation), Cloning, mammalian cell culture and transfection is required. Proficiency with culture of cell lines and primary cells is strongly preferred.
TemporaryExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Molecular biology (cloning, restriction digests) under the supervision of a senior scientist Protein science, including western blot Cell culture, sterile technique, and transfection.
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Education requirements include master's of science with specific focus in one or more of the following disciplines: cell signaling, cell physiology, protein biochemistry, cancer biology and immunology.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
Full-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Perform cell biology and molecular biology, including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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Biochemical techniques including quantitative Polymerase Chain Reaction (qPCR)/Digital Droplet PCR (ddPCR), Enzyme-Linked Immunosorbent Assay (ELISA), western blotting, gel-based assays, tissue culture, etc.
ExpandApply NowActive JobUpdated 3 days ago - UpvoteDownvoteShare Job
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Expert in ELISA techniques, flow cytometry, cell culture, immunoassays, antibody purification, and mouse handling, including immunizations, spleen removals, hybridomas, etc. These include intellectual input and decision-making on protein targets to be pursued, development of immunogens and immunization strategies, utilization of hybridoma technology, and antibody validation in vitro and in vivo.
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Roles & Responsibilities The Biochemist I is expected to work independently, perform all laboratory functions that involved in Tissue Culture such as Hybridoma roller bottle cell culture, ascites production, insect cell shaker culture, recombinant protein production, in-process assays and safety procedures.
$20Full-timeExpandApply NowActive JobUpdated 2 days ago - UpvoteDownvoteShare Job
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DESCRIPTIONUnder the supervision of the Principal Investigator (PI), the employee will assist in research on uncovering principles of cellular protein quality control by carrying out various biochemistry, cell biology and molecular biological procedures in mammalian cells and Zebrafish.
$21.39 - $23.06 an hourFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Collaborate with research, analytical development and process development teams to develop, qualify/validate AAV gene therapy cell-based biological potency assays, protein chemistry assays, and molecular biology assays for process monitoring and drug product release, specifically SDS-PAGE, western blot, droplet digital PCR or qPCR, culture of primary mammalian cells, and cell-based potency assays.
$84,000 - $118,667 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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This position will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA.
$57 - $60 an hourExpandApply NowActive JobUpdated 1 month ago - UpvoteDownvoteShare Job
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Strong expertise (4+ years) in mammalian cell culture, across a variety of plate and flask formats. We are looking for an enthusiastic and detail-oriented individual with a strong background in cell culture.
$90,000 - $120,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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The technician will be responsible for supporting research staff on mammalian tissue culture experiments by assisting with tasks such as tissue culture cell maintenance, plasmid DNA transfections, fluorescence imaging, cryo-em sample preparation, etc.
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Skillsets of interest include expertise in tissue culture, biochemical tools (SDS-PAGE, immunoblotting, ELISA, protein assay), and a good working knowledge of molecular techniques including generation of mutant plasmids, transfection with plasmids/siRNA for transient protein expression in cell-based reporter systems as well as use of CRISPR and transcriptomics/proteomics technologies.
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protein cell culture jobs in San Diego, CA
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