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Experience with multi-color flow cytometry, panel design, primary cell culture, cell sorting and/or data analysis is preferred. Develop in vitro cell-based assays (cell culture, gene editing, flow cytometry, high content imaging, cytokine profiling, cytotoxicity, proliferation, etc.
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The incumbent will provide knowledge, assistance and training in molecular biology and immunologic techniques and related data analysis methods, perform flow cytometry, microscopic imaging and related assays of immune tissues and cell functions, perform cell culture studies and studies in small animals (rodents), process human blood and tissues and assist in general lab functions.
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The ideal candidate will have a background in cell selection, lentiviral transduction, cell culture and expansion, and flow cytometry analysis. 6+ years of postdoctoral experience with PhD, 8+ years with MS, or 12+ years with BS in applicable scientific or engineering disciplines such as biotechnology, bioprocess, molecular biology, immunology, or biochemistry.
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Familiarity with the following cellular and biochemical readouts including immunofluorescence, luminescence, cell viability, FRET, ELISA, MSD, flow cytometry. Familiarity with the following cellular and biochemical readouts including immunofluorescence, luminescence, cell viability, FRET, ELISA, MSD, flow cytometry.
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Experience with relevant technologies with an emphasis on potency and functional assays, cell culture (e.g. aseptic techniques, cell line expansion), flow cytometry-based assays and ddPCR. Plan, coordinate and execute analytical testing of molecular assays such as qPCR/ddPCR and flow cytometry-based assays.
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These tasks may include molecular biology assays such as PCR and cloning; mammalian cell culture experiments; flow cytometry assays; isolating RNA and performing qPCR; and mouse genotyping and assays in mice.
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Experience with in vitro characterization including but not limited to multicolor flow cytometry, continuous (incucyte) killing assays, cytokine assays and mixed lymphocyte reactions is preferred.
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Excellent technical skills in the area of cellular immunology and molecular biology including cell culture, gene expression analysis, and multi-color flow cytometry. Excellent technical skills in the area of cellular immunology and molecular biology including cell culture, gene expression analysis, and multi-color flow cytometry.
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Develop and optimize flow cytometry-based assays for a range of models, including ex vivo cancer patient samples (hematologic and solid tumor), primary normal tissues, and in vitro cancer cell lines.
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We are seeking a highly motivated and talented Research Associate to join the flow cytometry core team within the Gilead Sciences Research department. The successful candidate will contribute to the day-to-day operations of the flow cytometry core and maintaining a high-functioning laboratory capable of meeting the needs of Gileads Oncology, Inflammation, and Virology therapeutic discovery groups.
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Proficient in MS Word, Excel, and PowerPoint, with knowledge related to scientific data analysis software for applications such as GraphPad Prism, JMP, NovoExpress/FCS Express (flow/spectral cytometry), and R (Seurat, sequencing.
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Experience performing flow cytometry, mass cytometry, cell sort (FACS), and knowledge using Diva, Flowjo, Cytobank, Cell engine, Omiqs, Graph-Pad Prism Bachelor’s degree in biological sciences, immunology, or chemistry with 2 years’ experience.
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Proficiency in multi-parameter flow and/or spectral cytometry including panel design, sample preparation and acquisition, antibody titration and data analysis. Experience in molecular and cellular biology techniques, including but not limited to ELISA based assays, Proquantum, Luminex and ddPCR.
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Must have experience in general cell based assays that include but not limited to: Primary cell culture, ELISAs, Westerns, qRT-PCR, Reporter Assays, Flow cytometry, transient and stable transfections.
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The successful candidate will contribute to the day-to-day operations of the flow cytometry core and maintaining a high-functioning laboratory capable of meeting the needs of Oncology, Inflammation, and Virology therapeutic discovery groups.
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histologist cytometry jobs in Palo Alto, CA
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