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Knowledge of and experience with basic cell and molecular biology protocols: tissue culture, PCR, DNA sequence analysis and cloning; comfort working with instrumentation and computers; maintenance of accurate notebooks and computer files; team player with good communication and interpersonal skills; willingness to learn, seek out advice and admit errors; highly motivated to succeed.
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Demonstrated skill in RT-PCR's, flow cytometry and immunological and molecular biology assays. Specific duties include tissue cell culture, flow cytometry, PCR, western blots, immunofluorescence, animal work, preparation of data for publication and grant proposals, ordering of supplies and equipment, upkeep of equipment and general lab maintenance.
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Assist in biochemical assays (such as western blotting, qPCR, tissue harvests, molecular cloning, immunofluorescence, histology) Experience with mouse and or zebrafish husbandry, molecular biology techniques, PCR, immunofluorescence, histology, microinjections.
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Prior experience with genome editing, vector construction, iPSC culture, primary human cell culture, small animal surgery, and/or tissue engineering. Candidates with a strong background in cell biology, molecular biology, biomedical engineering, genome editing, recombinant vector construction, induced pluripotent stem cell (iPSC) culture and differentiation, and/or skin biology are encouraged to apply.
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Carry out molecular biology techniques, including immunohistochemistry, DNA and RNA extraction, RT-PCR and real time RT-PCR. Experience with molecular biology, in vivo, and in vitro lab techniques.
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Ability to perform molecular biology techniques such as PCR, RT-PCR, cDNA synthesis, gene expression analysis, recombinant protein expression, RNA/DNA isolation, protein purification. Theoretical knowledge of molecular biology, immunology, cell biology, and/or biochemistry.
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Design and execute experimental procedures/protocols in research involving cell and molecular biology techniques, tissue culture, histology and immunological assay, polarizing and fluorescence microscopy, and animal model system including mouse breeding and genotyping.
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The successful candidate will perform cell culture studies, conduct in vivo experiments in mouse models, analyze mouse tissue at a molecular level, and present data. Perform PCR, Western blot, ELISA, immunostaining, immunohistochemistry, and other techniques of molecular biology as needed.
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Demonstrated hands-on experience in protein-based biomarker analysis and molecular biomarker analysis of biological samples. Support non-clinical necropsy and tissue processing. Support the development and validation of molecular fit-for-purpose clinical and non-clinical biomarkers following the FDA Bioanalytical Method Validation guidance.
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The individual will perform experiments supporting isolation of cells from airway tissue, molecular biology, cell culture of airway stem cells, differentiation of airway stem cells in air-liquid interface and animal work.
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Experience with lipid biochemistry, tissue and plasma lipid analysis, molecular biology techniques, and mammalian cell culture. The Department of Molecular Metabolism and the Harvard Chan Research Center on Causes and Prevention of Cardiovascular Disease (CAP-CVD) at the Harvard T.H. Chan School of Public Health invite applications for the position of Research Scientist.
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Biotechnology, biological science, sterile environment, sample collection, Histology, Pathology, Ascp, Immunohistochemistry, Tissue samples, Chemistry, Biology, biochemistry, Staining, Accessioning, Specimen processing, Sample preparation, embedding, sectioning.
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This includes cell-based assays, tissue culture, biochemical assay technologies, and protein and RNA expression analysis. Experience in cell biology, biochemistry, and/or molecular biology, in particular assay development and execution, is required.
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Work-related knowledge in tissue culture, molecular biology and genetics laboratory test techniques such as PCR, DNA/RNA extraction, cloning of cDNA sequences, amplification of DNA sequences by PCR, protein expression techniques, protein purification, plasmid analyses by restriction digest, protein gel electrophoresis, DNA gel electrophoresis, Northern blotting, Western blotting, Southern Blotting, siRNA/DNA transfections, are preferred.
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One of the nation's top Orthopaedic programs, the Department of Orthopaedic Surgery at the University of Pittsburgh conducts a wide spectrum of musculoskeletal research in areas of cell and molecular biology, developmental biology, stem cells and gene therapy, biomaterials, microphysiological systems, tissue engineering, and regenerative medicine.
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tissue molecular jobs Title: faculty position
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