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Perform various molecular and biochemical assays on tissue samples from preclinical in vivo studies for early discovery drug development programs, including quantitative and qualitative analysis of target mRNAs and proteins (tissue homogenization, RNA extraction, Western blot, ELISA, qPCR, dPCR.
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Additional duties include but are not limited to: hyperpolarized NMR spectroscopy and imaging, cell culture, DNA preparation, RNA isolation, protein purification and preparation, PCR-based genotyping, RT-PCR, Western Blotting, molecular subcloning, flow cytometry, immunofluorescence and live cell imaging.
ExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Background in molecular biology techniques such as cloning, site-directed mutagenesis, DNA and RNA isolation and purification, sequencing, RT-PCR, and qPCR. Rescue and characterize pseudoviruses and/or RNA viruses.
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Under general supervision performs complex laboratory techniques including, but not limited to recombinant protein purification, DNA purification, flow cytometry. A special advantage of this rapid-freezing method is that we can trap and image dynamic states of functioning macromolecular assemblies, such as open and closed states of membrane channels and viruses actively transcribing RNA. Three-dimensional density maps are obtained by digital image processing of the high-resolution electron micrographs.
ExpandApply NowActive JobUpdated 6 days ago - UpvoteDownvoteShare Job
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Specific Responsibilities:Molecular Biology:DNA, RNA, Protein isolation, purification and expression Western blottingPCR, RT-PCRPlasmid preparationCloning, mapping and sequencing of DNA Recombinant virus production and purificationCellular Biology:Cell culture, cell isolation Functional studies TransfectionImmunologyAntibody production and purificationAdministrationMaintains detailed records of results which may include collecting, extracting & entering data.
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Assist with ongoing projects in the laboratory which may involve performing the following activities: cloning, plasmid preps, PCR genotyping analysis, DNA and RNA extraction and purification, preparing samples for sequencing experiments, tissue culture, culturing of fungi and bacteria, microscopy, bioinformatics and/or data analysis.
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Experience with writing and following an institutional animal care and use committee (IACUC) protocols Experience with protein expression and purification, virus-like-particles production and concentration, and RNA vaccines Previous experience with immunization of mice and rabbits Advance skills in molecular biology methods, including cloning and PCR, tissue culture work with different cell lines, and transfection.
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You will be involved in developing and deploying high-throughput RNA production and purification platform assist in RNA production to ensure smooth operation. You will get to work on the cutting edge of biotech research in this exciting and exclusive role, working on the development and refinement of RNA constructs for a variety of therapeutic products.
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Support general lab activities such as: making solutions and buffers as needed and performing basic molecular cloning (primer design, PCR, DNA/RNA purification), as well as performing protein expression and protein purification and other molecular biology techniques.
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We are looking for candidates as Research Associate I or II, Oligonucleotide Chemistry to join our discovery chemistry team to advance cutting-edge RNA editing drug discovery programs. Research Associate I-II, Discovery Chemistry – Oligonucleotide (Purification.
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Strong molecular biology background with a proficiency in DNA/RNA isolation and purification, cloning, sub-cloning, PCR, RT-qPCR, genotyping, expression construct design, transfection, CRISPR gene editing, and RNA profiling.
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Experience and knowledge with neural cell culture, protein, DNA and RNA purification, animal surgery and mouse colony management. Experience and knowledge with neural cell culture, protein, DNA and RNA purification, animal surgery and mouse colony management.
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Proficiency with standard molecular biology techniques (RNA/DNA extraction, qPCR, RT-PCR, ddPCR) · Proficiency with protein purification and characterization (SDS-PAGE, blotting, enzymatic assays, etc.
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Experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots, and other standard cell-based or biochemical assays.
ExpandApply NowActive JobUpdated 5 days ago - UpvoteDownvoteShare Job
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Experience performing DNA, RNA, and protein preparation and purification is highly desired. Experience performing qPCR, qRT-PCR, DNA sequencing, ChIP-seq, mRNA isolation, northern blots, cDNA synthesis, immunoblots, co-immunoprecipitation, immunohistochemistry, and in situ hybridizations, are highly desired.
ExpandApply NowActive JobUpdated 4 days ago
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