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The candidate should be familiar with techniques conducted routinely in a human genetics and functional modeling laboratory such as molecular cloning, DNA and RNA extraction, DNA sequencing, gene expression studies, tissue culture or microscopy.
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Proficiency in molecular biology techniques such as DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, and Next Generation Sequencing. Proficiency in molecular biology techniques such as DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, and Next Generation Sequencing.
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Demonstrated experience of 3-5 years in executing a variety of cell biology techniques, including mammalian cell culture, in vitro cellular assays (e.g., cell viability, proliferation, invasion, apoptosis), and luciferase assay.
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Viral DNA, RNA and protein extraction, purification, and analysis, including cloning, amplification and DNA sequencing. Viral DNA, RNA and protein extraction, purification, and analysis, including cloning, amplification and DNA sequencing.
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Prior experience in microbial genomics assay implementation, including DNA/RNA extraction, library preparation, and high throughput sequencing (16S rRNA, ITS2, metagenomics, and/or dual RNASeq.
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Planning and executing experiments using genetics, molecular biology (PCR, restriction digest, gel electrophoresis, RNA extraction, qRT-PCR, CRISPR-based editing, DNA microinjections), basic biochemistry techniques (immunoprecipitations, Western blotting), behavioral experiments, fluorescence microscopy, and quantitative image analysis.
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Duties may include collecting tissue specimen from participants for DNA and RNA extraction, RNsequencing, and analysis. The ideal candidate for this position will have hands-on laboratory research experience and a working knowledge of molecular biology techniques (e.g. PCR, DNA extraction, sequencing.
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Perform, with technical independence, basic and advanced laboratory procedures such as Plasmodium falciparum tissue culture, genetic manipulation, nucleic acid extraction, PCR, qPCR, CRISPR-targeting design, and next-generation RNA and DNA sequence analysis.
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Core laboratory will also include the service of tissue preparation, extraction of various types of RNA and/or DNA, and transcription of RNA into complimentary DNA for downstream analysis.
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Utilize molecular biology techniques including DNA and RNA extraction, gel electrophoresis, and PCR to support product characterization. Execute routine analytical tests and quality control assays, such as qPCR, ELISA, SDS-PAGE, Western blot, and HPLC, to assess the purity, potency, and safety of AAV vector products.
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Basic molecular biology and microbiology techniques, such as aseptic technique, bacterial isolation and culture, strain library maintenance, PCR, DNA/RNA/protein extraction, cloning, recombineering, etc.
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Molecular biology techniques: DNA extraction from animal tissue, Genotyping, design of PCR primers, Western blotting, RNA isolation from animal tissue, restriction enzyme digestion and mapping, agarose gel electrophoresis, isolation of DNA from agarose gels, ligation of DNA, transformation of DNA, mini- and maxi-preparation of DNA, site-directed DNA mutagenesis.
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Experience in CLIA/CAP- processing patient samples- and manual molecular biology techniques, DNA/RNA extraction, qPCR, with pipetting at the very least. Current certification or eligible with ASCP (American Society of Clinical Pathologists) as an MLS - Medical Laboratory Scientist, MT - Medical Technologist, MLT-Medical Laboratory Technician, MB- Molecular Biology or specialist.
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Performs DNA/RNA extraction and purification, in vitro transcription, protein expression and purification, molecular cloning, and other standard molecular biology methods. Ability to execute standard molecular biology and biochemical methods, including PCR, molecular cloning, nucleic acid extraction and purification, gel electrophoresis, protein expression and purification, microbial cell culture, and preparation of media and buffers.
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Knowledge of biological sciences, general chemistry, and mathematics acquired through a Bachelor’s program in a biological field or equivalent degree with a strong background in anatomy and knowledge of appropriate collection techniques for downstream processes (histology, DNA/RNA isolation, protein extraction, etc.
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