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Solid technical ability and hands-on experience with molecular biology, ribonucleic acid ( RNA) and protein analysis, analytical gel electrophoresis, quantitative polymerase chain reaction (qPCR),enzyme-linked immunosorbent assay (ELISA), western blot, flow cytometry, quantitative reverse transaction (qRT-PCR), microscopy, histology, and cell culture.
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Avidity Biosciences, Inc.'s mission is to profoundly improve people's lives by delivering a new class of RNA therapeutics - Antibody Oligonucleotide Conjugates (AOCs™). Avidity is revolutionizing the field of RNA with its proprietary AOCs, which are designed to combine the specificity of monoclonal antibodies with the precision of oligonucleotide therapies to address targets and diseases previously unreachable with existing RNA therapies.
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Immuno-assays: western blot, ELISA, flow cytometry, immunofluorescence assay, immuno-histochemistry. Molecular cloning and basic DNA/RNA assays: molecular cloning, DNA/RNA isolation from cells, conventional PCR.
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Cell biology techniques: cell electroporation, cell transfection, cell surface antibody labeling, flow cytometry, dual-luciferase assays, preparation of DNA and RNA for deep sequencing. HIV work: Preparing HIV stock virus from transfected cell lines, titering HIV, challenging primary cells with HIV, challenging humanized mice with HIV via IP injection, collection of mouse blood containing HIV, quantification of viremia by qRT-PCR/ddPCR, flow cytometry, and ELISA.
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Molecular techniques including transfections, cloning, DNA/RNA extraction and PCR, restriction enzyme digestion. Common biochemical and immunological techniques, including SDS-PAGE, UV/Vis spectroscopy, ELISA and western blotting.
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Experience with standard molecular biology techniques strongly preferred (i.e. Protein/peptide sample prep and separation, RNA and DNA preparations, ELISA). Experience with standard molecular biology techniques strongly preferred (i.e. Protein/peptide sample prep and separation, RNA and DNA preparations, ELISA.
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Transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
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Prior laboratory hands-on experience with a variety of analytical techniques including, but not limited to ddPCR, nucleic acid purification/detection, protein quantification, ELISA, and gel electrophoresis.
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Based on our innovative approach to developing genetic medicines, as well as our industry-leading internal manufacturing capability and expertise, we have become the Astellas Center of Excellence for their newly created Genetic Regulation Focus Area. We are currently exploring three gene therapy modalities: gene replacement, exon skipping gene therapy, and vectorized RNA knockdown, with plans to expand our focus and geographic reach under Astellas.
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Expertise in in vitro transcription, analytical tools of RNA (gel electrophoresis, enzymatic reactions, immunoblotting, ELISA, qPCR and/or ddPCR) Perform multiple molecular and cellular biology techniques, including RNA transcription, tissue culture, cell transfection, qPCR, and other biochemical assays.
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The incubent will work alongside subject matter experts in the fields of cell biology and biomedical engineering, and perform functional and targeted assays to describe the neurobiology of the model systems, such as RNA isolation/sequencing, ELISA, and other in vitro assays.
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Flow cytometry and cell sorting, qRT-PCR and immunofluorescence analyses, ELISA. Our team is looking for an enthusiastic scientist to join our in vivo RNA delivery program. Familiar with ELISA, ELISpot, cytokine arrays.
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Experience with Next Generation Sequencing (NGS) technologies such as single-cell RNA seq. 1-3+ years of molecular biology wet lab experience (Western, PCR, ELISA, ELISpot, etc.) Experience with Next Generation Sequencing (NGS) technologies such as single-cell RNA seq.
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Knowledge of Luminex platform and working knowledge and experience in general cell biology, molecular biology techniques (PCR, qPCR, immunoblots, ELISA) You will also perform DNA/RNA/protein isolation/extraction/quantification from assorted biological material and tissue, as well as cell culture work, DNA/RNA/protein assays, research data procurement, and data analysis.
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Candidates with previous laboratory experience in DNA/RNA purification, PCR, ELISA, and Western Blot will be preferred. Conduct biochemical assays such as ELISA, enzyme activity and Western Blot.
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