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Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR. Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR.
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Strong background in molecular biology - DNA cloning, qPCR, RNA sequencing, Western blotting, ELISA, immunofluorescence, etc. Strong background in molecular biology - DNA cloning, qPCR, RNA sequencing, Western blotting, ELISA, immunofluorescence, etc.
Full-timeExpandApply NowActive JobUpdated 3 months ago - UpvoteDownvoteShare Job
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DNA, RNA, Protein isolation, purification and expression. ELISA, Elispot assay. The Northwestern campus sits on the traditional homelands of the people of the Council of Three Fires, the Ojibwe, Potawatomi, and Odawa as well as the Menominee, Miami and Ho-Chunk nations.
$26.27 - $36.95 an hourFull-timeExpandApply NowActive JobUpdated 3 months ago - UpvoteDownvoteShare Job
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Tech Work with all types of Molecular Biology techniques including: ELISA, DNA and RNA extraction, Polymerase Chain Reaction (PCR), Reverse Transcriptase PCR, Southern and Western Blotting, Gel Electrophoresis, Restriction Enzyme Digest, Capillary Electrophoresis, Sanger Sequencing, Probe Hybridization, Bead Capture Technology, and Microarray Technology.
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Experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots, and other standard cell-based or biochemical assays.
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Conduct various immunological assays such as ELISA, MSD, western blots, flow cytometry, and RT-qPCR. experiments (e.g. ELISA, MSD, flow cytometry). Hands on experience with mammalian cell culture, ELISpot, ELISA and Flow Cytometry is required.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA.
$57 - $60 an hourExpandApply NowActive JobUpdated 22 days ago - UpvoteDownvoteShare Job
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, Next Generation Sequencing, flow cytometry. Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, Next Generation Sequencing, flow cytometry.
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Develop, qualify, and validate (as appropriate) bioanalytical assays (expressions assays including mRNA and protein [ELISA/IHC], anti-AAV capsid nAb, CTL IFN-γ ELISPOT, IgG/IgM, biodistribution and shedding) for all programs and define science-driven, phase appropriate, and risk-based analytical development strategies to support the development candidate during discovery through preclinical evaluation (including GLP) and clinical development advancement.
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Design and execute lab experiments such as ELISA, in situ hybridization, DNA/RNA extraction, cDNA synthesis, ddPCR and blood processing. Design and execute lab experiments such as ELISA, in situ hybridization, DNA/RNA extraction, cDNA synthesis, ddPCR and blood processing.
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Some experience with some basic molecular biology techniques is a plus (e.g. ELISA, Western Blots, protein assays, cloning, RNA and DNA isolation). Dr. Wolf’s laboratory is looking for a highly motivated Research Associate II interested in the innate inflammatory responses of myeloid cells to join the Department of Biomedical Sciences and the Inflammatory Bowel Disease Institute (IBDI) at Cedars-Sinai.
$21.26 - $36.14 an hourExpandApply NowActive JobUpdated 8 days ago - UpvoteDownvoteShare Job
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Experience with assay development, Biomarker Analysis (dPCR, qPCR, Flow, Luminex, ELISA), DNA/RNA/protein analysis, cell-based potency assays, Experience with assay development, Biomarker Analysis (dPCR, qPCR, Flow, Luminex, ELISA), DNA/RNA/protein analysis, cell-based potency assays.
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Isolation of protein, RNA, and DNA from cultured cells and isolated murine ocular tissues with subsequent analyses; will involve numerous isolation kits as well as familiarity with standard methods for western blot, PCR, and ELISA assays.
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In addition, review and approve CTL assay development and validation reports, relevant sections of clinical documents pertaining to AAV and RNA analytical testing and documentation for submission to regulatory agencies.
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