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Will have a fundamental knowledge of fermentation and cell culture, protein chemistry and purification, analytical techniques and/or laboratory operations. Experience working with protein purification.
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Support antibody expression construct generation by molecular cloning, DNA sequencing, and plasmid DNA purification. We seek a motivated and collaborative team member with molecular cloning, protein expression, purification, and characterization experience to join our Redwood Shore site's Discovery Protein Science group.
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Has hands on experience with executing antibody or antibody-like molecule purification techniques including resin chromatography (affinity, CEX, AEX, MMC), depth filtration, virus filtration, membrane chromatography, and TFF operations.
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Assisting with molecular biology techniques such as cloning and plasmid purification, as well as isolation and analysis of RNA, DNA, and protein from mouse tissue and cellular samples. Assisting with biochemical techniques such as protein purification, enzyme assays, and metabolite measurements.
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Performs protein purification, cloning, and crystallography. JOB SUMMARY/ESSENTIAL JOB FUNCTIONS: The Research Technician 2 assists with conducting cloning, protein purification, and structural biology.
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Hands-on experience with analytical and purification techniques, including UV, NMR, HPLC, LCMS, and flash purification systems. Hands-on experience with analytical and purification techniques, including UV, NMR, HPLC, LCMS, and flash purification systems.
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Senior Associate Scientist, Purification Process Development - Pre-Pivotal Biologics - Foster City, CA. The Senior Associate Scientist, Purification Process Development (Pre-Pivotal) will lead and execute purification process development laboratory experimentation for Gilead biologics entities during pre-pivotal phases development (pre-clinical, phase I/II.
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The key function of this lab-based role is to lead multiple steps in the manufacturing of RNA, including DNA production, RNA in vitro transcription, RNA purification and RNA analytics to support key preclinical studies of new RNA therapeutics.
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Demonstration of expertise in column chromatography for protein purification and filtration technologies. The ideal candidate will have in-depth of knowledge of filtration, column/membrane chromatography, tangential flow filtration, other protein purification methods, and scale- up.
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Downstream purification of biologics including chromatography separations and filtration operations. fermentation and /or purification to develop robust, phase-appropriate processes for drug substance.
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Perform biochemical assays, protein expression and purification, assess sample quality, prepare samples for analytical studies included but no limited to spectroscopy (absorption, fluorescence, circular dichroism), determine binding constants by various methods (spectroscopic, analytical centrifugation, size-exclusion chromatography, SEC-MALS, DLS and related.
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Experience with common purification methods such as chromatography, filtration (depth filtration, UF/DF) and ultracentrifugation. The ideal candidate should have prior process development experience with common purification techniques such as clarification, chromatography, UF/DF and ultracentrifugation.
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Perform expression and purification of mono- and multi-specific antibodies and complex modalities fusion proteins from sub-milligram to gram scale to support antibody discovery, characterization, and pre-clinical validation of leads.
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Perform expression and purification of recombinant viral protein and human monoclonal antibodies and fragments ( E.coli, insect cells, mammalian cells) via affinity chromatography and FPLC-based size exclusion and ion exchange.
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Previous experience with methods in manufacturing of biologicals and protein purification. This position is responsible for providing on-the-floor Quality Assurance support functions for pharmaceutical compounding of tablets/liquids/suspensions/pastes, and pharmaceutical packaging operations by performing real-time batch record review, quality assessments, deviation decisions and Change Control activities.
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