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Initiating pre-purification and post-purification analysis of submitted samples by LCMS (liquid chromatography - mass spectrometry). This laboratory-based role will support their high throughput purification workflows by monitoring and maintaining instrument performance, executing sample handling tasks, and managing chemicals and consumables.
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Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable. Experience with recombinant protein expression and purification using mammalian cell lines and/or E. coli.
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Design, plan and execute multi-step organic synthesis and purification of high-quality target molecules and solve synthetic chemistry challenges to enable expansion of available chemical space. High levels of competence in all aspects of modern synthetic chemistry, including single compound synthesis, small parallel arrays, purification methods and analytical characterization.
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The ideal candidate will have hands-on experience working with mammalian cell culture, protein expression, purification and analytical QC using diverse biochemical methods such as ELISA, SDS-PAGE, Western blotting.
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Assisting with molecular biology techniques such as cloning and plasmid purification, as well as isolation and analysis of RNA, DNA, and protein from mouse tissue and cellular samples. Assisting with biochemical techniques such as protein purification, enzyme assays, and metabolite measurements.
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The purification group is responsible for non-GMP protein- drug lead generation, supporting the various research units and their pre-clinical therapeutic in- vitro and in- vivo needs. General knowledge on protein purification, protein conjugation, HPLC analysis, SDS-PAGE analysis and mass spectrometric analysis of protein.
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Skills: HPLC, SOP, In Vitro, In Vivo, Protein Purification, GMP (Good Manufacturing Practice) The purification group is responsible for non-GMP protein- drug lead generation, supporting the various company research units and their pre-clinical therapeutic in- vitro and in- vivo needs.
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The key function of this lab-based role is to lead multiple steps in the manufacturing of RNA, including DNA production, RNA in vitro transcription, RNA purification and RNA analytics to support key preclinical studies of new RNA therapeutics.
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Job Description: BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA. Qualified candidate will play an essential role in a high-energy DNA production and protein expression research laboratory.
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Downstream purification of biologics including chromatography separations and filtration operations. fermentation and /or purification to develop robust, phase-appropriate processes for drug substance.
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This individual will be responsible for building an internal oligonucleotide chemistry lab and will lead the design, synthesis, purification, and analysis of oligonucleotides and their conjugates.
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Basic molecular biology including plasmid construction, nucleic acid purification, DNA sequencing, and PCR. Basic molecular biology including plasmid construction, nucleic acid purification, DNA sequencing, and PCR.
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Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is preferred. Experience with recombinant protein expression and purification using mammalian cell lines is preferred.
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The Separation Sciences group in Discovery Chemistry at our client in Boston, MA is seeking a candidate to join their team whose mission is to analyze and purify novel medicines prepared by their medicinal chemist colleagues.
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Downstream AKTA Protein Purification & Chromatography experience in GMP environment. Develop and optimize downstream processes for the purification of therapeutic proteins, ensuring high yield, purity, and product quality.
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purification job in Boston, MA
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