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In this role, you will work as part of the QC team and responsible for effectively managing the QC process of Takara’s Reproductive Health Technology (RHT) and Next Generation Sequencing (NGS) product portfolio.
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NGS (next generation sequencing ) data formats (e.g. Bam, Fastq,) and Data pipelines (WGS, Broad GATF, nf-core. Wet Lab Process engineering - lab assay data collection, QC, Process monitoring, ELNs.
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Illumina library preparation, QC (Bioanalyzer, qPCR), and NGS sequencing. Sample preparation for Single Cell assays, including cell culture, tissue dissociation, cell enrichment, flow cytometry, and nuclei isolation.
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Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations. Conducting routine DNA characterization and quality control testing using techniques such as gel electrophoresis, fragment analysis, nanodrop, endotoxin testing, and preparation of samples for sequencing analysis (Sanger and NGS.
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The primary purpose of the Associate Data Scientist position is to provide bioinformatics analysis of next generation sequencing (NGS) data for faculty across the institution, to process NGS data for Advanced Technology Genomics Core facility, to perform quality control (QC) analysis and to maintain QC metrics and sample records of NGS experiments on the servers, and to meet with faculty to review data analysis results.
Full-timeExpandApply NowActive JobUpdated 6 days ago - UpvoteDownvoteShare Job
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Perform all basic QC assays that are part of sample preparation processes for NGS, including, but not limited to: Fluorometric assays (on the QUBIT and FLUOstar Omega BMG), size selection protocol (on the SAGE-ELF and gels, magnetic bead purification (AMPure), and DNA fragmentation protocols (Covaris S220 and Covaris E220.
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Hands-on experience with NGS and DNA/RNA molecular biology is required. We aim to find the best talent to join us and contribute to this exciting endeavor as a critical member of our QC team.
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Familiarity with Sequencing such as NGS or Sanger. Conducting routine molecular biology techniques including cell transformations, day cultures, bacterial cultures, Gibson assembly, and digestions.
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Develop, implement, and validate bioinformatics pipelines, including data processing, QC, analysis, and interpretation of NGS data. Proficiency in genomics, sequence analysis, and secondary analysis, such as: variant calling (e.g., SNVs/MNVs/indels, CNV, structural variants), NGS QC, splicing, circular nucleic acids.
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Perform Next Generation Sequencing (NGS) library (e.g. amplicon-seq and GBS) preparation/QC for Illumina sequencing to support high-throughput genotyping. Demonstrable skills in NGS library preparation and Illumina sequencing technology.
Full-timeExpandApply NowActive JobUpdated 15 days ago - UpvoteDownvoteShare Job
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The Senior QC Scientist is an on-site role located in San Jose, CA.How you will make an impact:In this role, you will work as part of the QC team and responsible for effectively managing the QC process of Reproductive Health Technology (RHT) and Next Generation Sequencing (NGS) product portfolio.
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Evaluate sequencing QC metrics for various standard Next-Generation Sequencing (NGS) workflows (RNseq, amplicons, WGS, ATAC-seq, etc.) Experience with workflow engines common in NGS processing (Toil, Airflow, CWL, etc.
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Experience in library construction for NGS, with emphasis on Process Development and optimization of diagnostic assays in a CLIA or FDA regulated laboratory environments is highly desired. Previous experience working in Assay Development or QC lab is highly desired.
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Two years of documented hands-on (wet lab) experience with NGS assay development, validation, troubleshooting, and reviewing of run QC data preferred. The Lead Research Scientist, NGS Core will also be responsible for work in the laboratory, performing sequencing, and analyzing sequencing data.
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Perform Next Generation Sequencing (NGS) library preparation/QC. Assist in developing SNP panels and associated assays for various species [emphasis on Next Generation Sequencing (NGS) applications on Thermo ION platform and Oxford Nanopore platform.
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