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Mammalian cell culture, molecular biology/cloning, PCR, and other common biology techniques like western blotting, flow cytometry, microscopy Familiarity with automatic, robotic dispensers, liquid handlers Strong problem-solving skills and the ability to work independently as well as part of a team Strong written and oral communication skills.
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Strong background and in-depth knowledge in analytical chemistry e.g. Flow Cytometry, ELISA, HPLC, CE, PCR, etc. As a Senior Member of the team the QC Analytical Scientist will be involved in various QC projects and should have a deep understanding of general QC flow and the Product Cycle.
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Highly experienced in protein assay development such as western blot/JESS, flow cytometry, ELISA, AlphaLisa, and immunoprecipitation. Develop other molecular biology-based assays using techniques such as cloning, DNA/RNA/protein extraction and RT-PCR.
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Doing basic imaging and flow cytometry to analyze expression. Experience with flow cytometry or imaging. Proficiency in PCR methods, DNA Binding, Western Blotting, ELISA, ChIP, immunostaining, and multiplexed protein assays.
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Hands-on experience with ex vivo analysis employing contemporary cellular, molecular, and immunological techniques such as PCR or NanoString, flow cytometry, and protein analysis by Western/MSD/ELISA etc.
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These tasks may include molecular biology assays such as PCR and cloning; mammalian cell culture experiments; flow cytometry assays; isolating RNA and performing qPCR; and mouse genotyping and assays in mice.
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Experience with a variety of molecular and cellular biology techniques, such as molecular cloning, DNA/RNA/Protein purification, PCR including ddPCR and qPCR, flow cytometry, Western blotting, ELISAs, bioassays, plate-based luminescence/colorimetric assays etc., with the ability to learn, troubleshoot, and execute for the goals of the team.
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HIV work: Preparing HIV stock virus from transfected cell lines, titering HIV, challenging primary cells with HIV, challenging humanized mice with HIV via IP injection, collection of mouse blood containing HIV, quantification of viremia by qRT-PCR/ddPCR, flow cytometry, and ELISA.
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DNA Extraction and PCR including but not limited to Next Generation Sequencing as well as Flow Cytometry and Antibody testing experience desired. DNA Extraction and PCR including but not limited to Next Generation Sequencing as well as Flow Cytometry and Antibody testing experience desired.
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Immunology: ELISA; Cytokine assay; Flow cytometry. Molecular Biology: DNA, RNA, and protein extraction; cloning; PCR; qRT-PCR; recombinant virus production and purification.
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Must have experience in general cell based assays that include but not limited to: Primary cell culture, ELISAs, Westerns, qRT-PCR, Reporter Assays, Flow cytometry, transient and stable transfections.
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Preferred qualifications: Familiar with techniques such as multi-color flow cytometry and/or mass cytometry, sterile technique for cell culture with BSL-2+ practices, ELISA, qRT-PCR, and sterile technique for bacterial culture.
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With respect to laboratory technical skills, the individual must be proficient in cell/tissue culture, flow cytometry/sorting (FACS), protein isolation and analysis (western blot, ELISA, and.
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Experience with flow cytometry, PCR, and cloning is preferred. Specific duties include tissue culture, flow cytometry, in vivo murine work, epigenetic profiling, single cell and next generation sequencing, and other basic molecular biology techniques.
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Flow cytometry and cell sorting, qRT-PCR and immunofluorescence analyses, ELISA. Expertise in multicolor flow cytometry and cell sorting, fluorescent and confocal microscopy.
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