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Techniques include: high parameter flow cytometry (up to 27 colors), in vivo mouse work, fluorescent microscopy, confocal microscopy, standard tissue culture, standard mouse handling techniques, ELISA, ELISpot, 10x genomics, single cell PCR.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linked immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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It is preferred for the candidate to have experience in some or all of the following: immunology/molecular biology assays (e.g. ELISA, flow cytometry, PCR), mouse handing, monitoring, husbandry, surgical and non-surgical animal procedures, and analysis of sleep, otherwise the candidate must be willing to learn these techniques.
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Deep knowledge and experience in various analytical methods employed for cell therapy products, including flow cytometry, molecular/PCR-based assays, cell-based potency assays, and safety testing.
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Analysis of flow cytometry results will be done using FlowJo or Cyflogic programs and involves analysis of fluorescence readouts, live/dead cells discrimination and multi color compensation.
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Preferred QualificationsWork Experience:Experience in molecular biology, transfection, gene expression, PCR, Q PCR, arrays), biochemistry, immunoblotting, flow cytometry, and/or protein-protein interaction.
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Knowledge/experience with (or willingness to learn from cheerful PI and lab members): FACS techniques including cell staining, flow cytometry, and cell sorting, molecular biology, including DNA cloning, PCR and RNA handling, virology, histology, mouse handling desired but not required.
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TASKS. The contractor shall: Perform a wide range of complex procedures and techniques including cell culture, molecular biology, DNA/RNA analysis, DNA/RNA isolation, DNA/RNA purification, DNA/RNA next generation (Illumina) sequencing, flow cytometry, and gene cloning.
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Highly experienced in protein assay development such as western blot/JESS, flow cytometry, ELISA, AlphaLisa, and immunoprecipitation. Develop other molecular biology-based assays using techniques such as cloning, DNA/RNA/protein extraction and RT-PCR.
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Under the general supervision of the investigator, performs complex laboratory experiments in the field of cell biology including: cell culture, ELISA, DNA/RNA isolation, PCR, general biochemistry, immunohistochemistry, flow cytometry, and microscopy.
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Biochemistry and protein chemistry experience (flow cytometry, FACS, ELISA, western blotting, SDS-PAGE, etc.) Designs and executes cell-based and biochemical assays to validate engineered cell lines and targets through flow cytometry, reporter assays, ELISA, western blotting, and cytotoxicity assays to select lead preclinical candidates.
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Independently perform cellular and molecular biology assays including: flow cytometry, ELISA, PCR, and immunocytochemistry. Correctly use and maintain laboratory equipment such as micro-plate reader, balances, centrifuges, microscopes, pipettes, cryostat, and laminar flow hoods.
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Molecular biology/cloning, PCR, and other common biology techniques like western blotting, flow cytometry, microscopy, and biochemical assays. Molecular biology/cloning, PCR, and other common biology techniques like western blotting, flow cytometry, microscopy, and biochemical assays.
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The ideal candidate will have a working knowledge of immunology and prior technical experience with flow cytometry, ELISA, quantitative real-time PCR, and data analysis/visualization software.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry) · Experience with high-throughput drug screening is a plus · Possess some trouble shooting and problem-solving skills to resolve technical questions in a multi- disciplinary environment.
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