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The position will support experiments using DNA, RNA/transcriptomics, RT-PCR, protein/westerns and immunofluorescence/confocal approaches for molecular mechanistic and translational toxicology research.
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Experience in assay development, mammalian cell culture, ELISA, Western blotting, qPCR, and experience in standard molecular biology techniques such as cloning, protein expression and purification is preferred.
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The Research Associate I/II will be responsible for executing bioanalytical assays (including ELISA, MSD, and qPCR) to support non-clinical development of Beam's drug candidates. Bachelors degree in scientific field (Biochemistry or Molecular Biology preferred.
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Experience with mammalian cell culture, and in standard molecular biology techniques such as cloning, DNA purification, and protein expression is preferred. Experience in performing bio-analytical methods (immunological, microbiological, molecular biological, or virological) following defined processes.
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Experience with immunoassay techniques including IF, flow cytometry, and ELISA to support in vitro platform development studies. Familiarity and hands on experience with analytical techniques such as Immunofluorescence, Western blotting, ELISA and flow cytometry-based assays.
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Perform laboratory testing such as ELISA, Luminex Cytokine Analyses, acrophage phagocytosis assays, bacterial burdens in organ tissue, DNA chromosomal preparation, PCRs, isolating and purifying plasmid DNA specimens, and Agar gel analysis.
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Daily tasks will involve cell culture (primary cultures and cell lines), Western blot, immunofluorescence, and ELISA. Additional duties may include equipment maintenance, preparation of stock laboratory reagents, and maintaining an organized laboratory notebook.
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Required) Skills with techniques such as PCR, transfection, RNA/DNA extraction, Western blotting, immunoprecipitation, immunofluorescence, using radioactive materials safely, SDS-PAGE, ELISA based assays, fluorescence microscopy, expression of active proteins in several systems, etc.
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Human microbiome and immunopathogenesis studies flow cytometry, ELISA, qPCR). Additional Training and Experience Requirements: Experience of doing experiments related to microbiology and immunology or molecular biology.
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Perform serologic, molecular and immunologic procedures that may include, but are not limited to, DNA extraction, PCR amplification, sequence-based typing, STR analysis, probe hybridization/detection, cytotoxic crossmatching, antibody screening/identification by Luminex and/or ELISA assays and other procedures as defined.
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You will have the opportunity to learn about various cell and molecular biology techniques like cell culture, microbiome sequencing, fluorescence microscopy, ELISA, and qPCR. Throughout the internship, you will be part of our challenging and engaging projects and focus on developing research and presentation skills and problem solving skills.
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Specific Experience: Individual most possess comprehensive knowledge and experience in molecular biology techniques including but not limited to cell culture, RT-PCR, western blotting, nucleic acid isolation and purification, basic histology, and ELISA design and performance.
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Expertise in tissue culture and immunological/virological techniques, including aseptic techniques, cell line maintenance, production of plasmids and virus-like particles, Western blot, ELISA, ELISPOT, Luminex assays, flow cytometry, virus neutralization assays and PCR.Ability to obtain and maintain a security clearance.
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Overview:As a Research Scholar, you will operate under the guidance of a Senior Scientist, contributing to the optimization and validation of various molecular assays aimed at detecting quarantine pathogens, particularly plant viruses, viroids, bacteria, and fungi.
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Cell culture, immunohistochemistry, molecular assays, Western blotting, protein purification, ELISA, etc) and synthesizes and interprets results. cell culture, immunohistochemistry, molecular assays, Western blotting, protein purification, ELISA, etc) and synthesizes and interprets results.
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