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Role requires agility and flexibility in the application of diverse cellular and molecular biology techniques and assays (e.g. cell/organoid culture, compound screening, FACS, ELISA, MSD, western blotting, qRT-PCR, cell transfections & transductions, viral productions etc.
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Expert in ELISA techniques, flow cytometry, cell culture, immunoassays, antibody purification, and mouse handling, including immunizations, spleen removals, hybridomas, etc. These include intellectual input and decision-making on protein targets to be pursued, development of immunogens and immunization strategies, utilization of hybridoma technology, and antibody validation in vitro and in vivo.
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The Medical Research Specialist is responsible for performing highly specialized virology and immunology research pertinent to various TNPRC AIDS vaccine research projects, which includes conducting a wide range of molecular, virological, immunological and cell culture techniques and laboratory assays.
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The individual in this position will assist with specific procedures, such as tissue processing and staining for flow cytometry, in vitro mammalian cell culture, and aerobic and anaerobic bacterial culture.
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Conducts radioligand binding, biochemical and cell-based signaling assays to support the Internal Medicine Research Unit, including in vitro and ex vivo experiments to help characterize novel targets, characterize target engagement and downstream effects of small molecule modulators.
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Bachelor’s degree in cell biology or Bioengineering with at least three years of experience involving aseptic primary human cell culture and biology techniques, such as ELISA, qPCR, microscopy or related experience required.
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The candidate must be skilled in a wide variety of state-of-the-art molecular biology techniques, human pluripotent stem cell culture, and highly experienced in gene editing technologies, and in the development of in vitro differentiation methodologies, especially in the area of hematopoiesis.
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The company provides unrivaled service and quality to scientists working in biopharmaceuticals, cell therapy, and regenerative medicine, assisted reproductive technology and cytogenetics, and industrial cell culture for the large-scale production of biotherapeutics and vaccines.
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The company provides unrivaled service and quality to scientists working in biopharmaceuticals, cell therapy, regenerative medicine, assisted reproductive technology and cytogenetics, and industrial cell culture for the large-scale production of biotherapeutics and vaccines.
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Preferred skills include Cell culture, Virus culture, Bacteriology, Protein purification and conjugation, Microscopy, experience with Laboratory equipment including Spectrophotometer pH meter, centrifuges, column chromatography, and performing diagnostic assays.
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Support troubleshooting and optimization of cell culture and bioassays such as in-vitro potency. The successful candidate will have hands-on experience with cell-based in-vitro potency assays, SDS-PAGE, Automated Western blot assays, enzyme activity measurements, protein extraction, purification, and quantification methods.
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Generates plasmids for producing lentiviruses to manipulate gene expression in cell culture (shRNA, CRISPR). Duties will include cell culture, cloning, virus generation and cell transformation, as well as some animal work.
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Performs cell culture of tumor cell lines and 3D tissue-engineered cultures based on polymeric scaffolds for organotypic tumor growth in vitro. Optional: knowledge on CAD design of hardware, basic knowledge of cancer cell biology.
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In vitro cellular assays, mammalian cell culture, Lentiviral Transduction, 2D/3D cell culture, SDS-PAGE/Western blot, qPCR, CRISPR, Luciferase, CellTiter-Glo, TR-FRET/BRET, MSD, GraphPad Prism, Genedata Screener, R, immunofluorescence, luminescence, cell viability, FRET, ELISA, MSD, flow cytometry.
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3-5 years of experience performing cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay.
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