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Proficient in molecular and cell based analytical methods including cell culture, In vitro cell based assays, ddPCR, ELISA, and flow cytometry. Maintain up to date knowledge of cell-based assay and potency assay development requirements especially in the areas of gene therapy for neuromuscular and cardiac indications.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Experience with in vitro and in vivo pharmacology studies, e.g. sterile cell culture techniques, and rodent handling experience (animal dosing (IV, PO, IP, SC), tumor implantation, blood/tissue collection, etc.
$20.84 - $27.22Full-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Lead the cultivation and expansion of mammalian cells, overseeing the maintenance of in vitro cell culture studies. Proven expertise in cell culture techniques and a high level of proficiency in molecular biology techniques, including ELISA and PCR.
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Work with the team to select indications, design, plan, and execute in vitro and in vivo experiments to select constructs, establish dose response relationships in animal models and cell culture systems.
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Demonstrated experience of 3-5 years in executing a variety of cell biology techniques, including mammalian cell culture, in vitro cellular assays (e.g., cell viability, proliferation, invasion, apoptosis), and luciferase assay.
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This position additionally requires providing technical support for in vitro and in vivo experiments, encompassing molecular biology, cell culture, and flow cytometry, for the benefit of post-doctoral fellows and graduate students within the laboratory.
$43,919 - $81,000 a yearFull-timeExpandUpdated Today - UpvoteDownvoteShare Job
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The specialist will perform in vitro cell culture assays, qRT-PCR, molecular biology cloning, CRISPR gene editing, biochemistry, and next generation sequencing assays including single cell genomics.
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Experience in tissue culture (maintenance of transformed and primary cells), In vitro cell-based assays is preferred. In vitro cell based assays. Working with multiparameter flow cytometry, including cell surface staining, intracellular staining, data acquisition and analysis.
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Integrate diverse data sources into model development and optimization (in vitro cell culture, immunoassays, clinical studies, large-scale omics data, etc) PhD or Master’s degree in mathematics, computational biology, biomedical engineering, or related field.
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Knowledge and experience in immunology, cell culture, flow cytometry, and in vitro methods, including experience setting up a new laboratory, is highly desired. The Senior Scientist serves as Study Director and is responsible for the oversight and conduct of in-vivo studies involving the testing of pharmaceutical compounds and cell and gene therapy.
$100,000 - $120,000 a yearFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Develop in vitro cell-based assays (cell culture, gene editing, flow cytometry, high content imaging, cytokine profiling, cytotoxicity, proliferation, etc.) Experience with multi-color flow cytometry, panel design, primary cell culture, cell sorting and/or data analysis is preferred.
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Strongest consideration will be given to candidates with expertise in molecular cloning, nucleic acid chemistry, in vitro transcription, mRNA analytics, protein expression analysis, and basic cell culture.
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The successful candidate should have laboratory experience in most or all of the following fields: mammalian cell culture (adherent and suspension), molecular biology techniques (PCR, sequencing, restriction mapping), genome analysis, bioanalysis (e.g. FACS, ELISA, assays of immune cell function), and inhaled product development.
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Experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots, and other standard cell-based or biochemical assays.
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