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Develop and perform cellular and molecular assay techniques (qPCR, dPCR, ELISA, flow cytometry) Familiarity with diverse analytical techniques such as Western Blot, ELISA, qPCR. Develop and perform cellular and molecular assay techniques (qPCR, dPCR, ELISA, flow cytometry.
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Previous experience using JMP for Design of Experiments to identify and optimize critical assay parameters, estimating shelf-life, determining method capability, and running statistical analyses.
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Experience in analyzing biologics including monoclonal antibodies and/or other therapeutic proteins in one or more of the following techniques: ELISA (HCP, proA) and binding potency assay.
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Adverum’s core capabilities include clinical development, novel vector discovery and in-house manufacturing expertise, specifically in scalable process development, assay development, and current Good Manufacturing Practices quality control.
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12 monthsLocation: Exton, PAAs a Quality Analyst, your responsibilities:Description: The QC Analyst 2 will contribute to the success of the operation by providing accurate, GxP-compliant analyses of clinical biopharmaceutical products and by participating in assay qualification and validation studies.
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Experience developing and applying biological characterization methods (e.g. cell-based assays, BLI or other protein binding kinetic assays, ELISA, and homogeneous assay formats such as AlphaLISA.
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We are seeking a highly motivated and talented scientist with background in cellular and molecular biology and assay development to join the Company’s Biotherapeutics and Genetic Medicine organization to support in vitro pharmacology work related to Genetic medicine research on AAV.
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Broad technical expertise/knowledge including with flow cytometry, ELISA, immunohistochemistry, qRT-PCR, NGS and RNA Seq. Prior management of contract research organizations (CROs) for clinical biomarker assay development and qualification.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA.
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Must have experience with Fluorescence, ELISA, sandwich ELISA, Gel Electrophoresis Must have some statistical analysis experience, JMP or R is a plus. Must have experience with assay optimization.
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The incumbent will be responsible for drug screening, cell-based assay development, and biomarker discovery. Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
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Experience with standard immunological assays such as flow cytometry / FACS, magnetic cell sorting, ELISA/Luminex, immunofluorescence staining and confocal microscopy, cell proliferation assays, cytotoxicity assays.
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Immunology: ELISA; Cytokine assay; Flow cytometry. The Research Specialist will actively participate in biobanking activities and contribute to collaborative translational research in transplant immunology by using techniques of cellular and molecular biology, biochemistry, immunology, and proteomics, as well as maintaining the day-to-day needs of the lab.
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Dependent upon what area of the laboratory the technologist shall work, the technologist shall be proficient in assays/methodologies including, but not limited to: ABO typing, Nucleic Acid Testing, serological assay (EIA, ELISA) and Histocompatibility testing.
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Other analytical techniques such as ELISA, HPLC, ELISPOT assay. BS or MS in Biology, Molecular Biology, Biochemistry, or a related discipline with 2+ years laboratory research experience.
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