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You have experience with cell line engineering and validation (e.g. stable retroviral transduction, transient DNA/mRNA transfection, CRISPR) You have experience with primary human immune cell activation assays using multparameter flow cytometry.
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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Under the supervision of the principal investigator and in cooperation with other lab members and senior scientists in neighboring labs, the Research Associate will design and conduct experiments using a variety of skills, which may include but are not limited to: microscopy, live cell imaging, quantitative cell biology, molecular biology, computational analysis, and the production and use of microfluidic devices.
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In health, we focus on areas like medical countermeasures, advanced therapeutics, regenerative medicine, cell and gene therapies, biologics manufacturing, natural products, APIs and KSMs, enzymes, biocatalysis, and microbiome therapy to support national health security and public health initiatives.
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The major focus of this position will be the in vivo evaluation of novel cell and genetic therapies, biologics and small molecules across multiple programs to support programs from target validation to identification of clinical candidates.
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An ideal candidate should have a strong background in immortalized (hepatocytes) and primary cell culture (HSC, T cells) and have experience screening in these cell types using various CRISPR delivery systems, including RNPs and LNPs.
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Analysis of blood and tissue samples using a variety of techniques, including flow cytometry, ELISA, Luminex and other molecular and cell biology techniques. Experience with characterization and assay of immune cell population and cytokines is desired, but not required (e.g., Multicolor FACS, Luminex, ELISA, etc.
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Helps to produce and isolates samples for proteomics studies using cell culture techniques, immunoprecipitation, subcellular fractionation methods and protein sample preparation from tissues and/or biofluids.
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The candidate is expected to have a firm grasp of human T cell biology and the design and analysis of complex multicolor flow cytometry endpoints. The company is developing two distinct and complementary platforms: a potent, highly selective cell-targeted lipid nanoparticle (ctLNP) delivery system and a novel immune-quiet DNA (iqDNA) cargo produced by a scalable capsid-free manufacturing process that uses proprietary cell-free rapid enzymatic synthesis (RES.
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Expression and purification of antibody constructs in mammalian cell culture at micro, medium and large scale as appropriate. BA in Molecular Biology or Protein Engineering and 4+ years industry experience in biologics discovery and protein science OR MSc and 2+ years experience.
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We are seeking a highly motivated and talented Research Associate/Senior Research Associate to join our Oncology Cellular Therapy Group to advance our efforts in induced-pluripotent stem cell (iPSC)-derived immune cell therapies.
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Host Cell Proteins, residual ProA, DNA) in Biologics. Support in the lab or oversee characterization of critical reagents (Antibodies, conjugated antibodies, assay controls, calibration standards) using a variety of fundamental biochemical methods including ELISA, 1 and 2 D gel electrophoresis methods, western blot, HPLC and LCMS methods and protein concentration determination.
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Its patent-pending and proprietary pluripotent stem cell differentiation technology platform TFomeTM was developed in Professor George Church’s lab, a pioneer in synthetic biology, and allows for the development of unique cell therapies with significantly streamlined manufacturing, improved cell quality, efficiency and speed.
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Expertise/competence in analytical techniques for biologics such as HPLC, CE-SDS, icIEF spectroscopy, ELISA, cell-based bioassays etc. A Third Rock Ventures Stealth NewCo is seeking a Senior Manager/Associate Director, Quality Control (QC) to join their growing, experienced, and collaborative CMC team.
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The project involves the handling and analysis of human fallopian tube tissue for imaging, cell harvesting, and other downstream applications. RESEARCH SUPPORT ASSOCIATE , Koch Institute (KI) for Integrative Cancer Research- Belcher Lab , to join an ongoing research project funded via a radical collaboration research mechanism through Break Through Cancer, a five-way consortium between KI, Johns Hopkins Medicine, MD Anderson Cancer Center, Memorial Sloan Kettering Cancer Center, and the Dana-Farber Cancer Institute.
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cell job Title: associate in Waltham, MA
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