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Transient cell culture techniques such as transfection, cell number and viability monitoring, cell lysis and protein harvest Purification of proteins using liquid chromatography techniques.
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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Proficiency in a broad range of biochemical, molecular and cellular techniques including (but not limited to): mammalian cell culture, western blotting, qPCR, transfection, flow cytometry etc.
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Trained in sterile technique including hands-on experience with mammalian cell culture. Culture, maintain, and manipulate human primary cells and cell lines in standard culture and on novel Draper platform technologies.
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Required Skills Bachelor's Degree - Biology or related field with 2-4 years of experience High level of technical expertise in some of these relevant areas: cell culture, protein/RNA/DNA analyses, in vivo animal studies (behavior, dosing, sample collection, stereotaxic surgery, etc.
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Strong hands-on experience in mammalian cell culture including expertise in aseptic technique, culture optimization, cell counting, cryopreservation, and validation methods.
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Cell culture maintenance, cryopreservation, validation, and inventory management. Proficiency in experimental design, data analysis, and a range of biology skill sets including in vitro cell assay development, plate-based assays, Western blotting, protein quantification, ELISA, MSD, transfections, lentiviral transductions, siRNA/shRNA/CRISPR, qPCR, and viability assays.
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Job Description: The Mechanism of Cancer Resistance (MoCR) Thematic Research Center (TRC) is seeking a highly motivated Associate Scientist to support Discovery Biology research programs at our facility in Cambridge, MA. The ideal candidate will contribute to our discovery pipeline from the bench by taking responsibility for developing, optimizing, and troubleshooting cellular assays to characterize and advance Client molecules of interest.
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The Mechanism of Cancer Resistance (MoCR) Thematic Research Center (TRC) is seeking a highly motivated Associate Scientist to support Discovery Biology research programs at our facility in Cambridge, MA. The ideal candidate will contribute to our discovery pipeline from the bench by taking responsibility for developing, optimizing, and troubleshooting cellular assays to characterize and advance novel molecules of interest.
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Experience with mammalian cell culture, molecular biology techniques such as DNA and RNA extraction, qPCR, protein quantification, ELISA, and enzymatic assays is required. We are seeking a talented and highly motivated Sr. Associate Scientist in molecular biology and metabolism to support our drug discovery programs.
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The Mechanism of Cancer Resistance (MoCR) Thematic Research Center (TRC) is seeking a highly motivated Associate Scientist to support Discovery Biology research programs at our facility in Cambridge, MA. The ideal candidate will contribute to our discovery pipeline from the bench by taking responsibility for developing, optimizing, and troubleshooting cellular assays to characterize and advance Client molecules of interest.
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Maintain mammalian cell culture and develop screening capabilities for quantitative analysis of on-target inhibitor activity in tumor cells (e.g., anti-proliferation, target engagement, RNA modulation, shRNA/CRISPR based methods of gene loss.
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Bachelor’s degree in Biology, Molecular/Cell Biology, biochemistry or a related discipline, with 4+ years of relevant work experience. Experience in managing or supporting cell banking, and familiarity with databases such as Mosaic, would be a plus but not required.
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Working both independently and collaboratively to support cutting-edge Oncology drug discovery biology programs. The ideal candidate will thrive in a collaborative environment and make meaningful contributions as a valued member of our dynamic drug discovery team.
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Immediate need for a talented Associate Research Scientist. Develop and execute mechanistic in vitro cellular assays to characterize mechanism of action of Client compounds. Immediate need for a talented Associate Research Scientist.
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cell culture jobs Title: associate scientist in Waltham, MA
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