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Previous laboratory animal experience and being comfortable working with rodent and primary tissues (rodent and non-human primate). Support animal study in compliance with Sanofi policies - Basic rodent manipulation and techniques (including but not limited to a range of various blood collection methods, necropsy collections, and tissue processing for flow analysis, etc.
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Experience with vivarium responsibilities including non-clinical ocular imaging, animal handling, intravitreal injection, eye dissection, perfusion is required. Basic wet lab skills, including but not limited to, molecular biology and biochemistry techniques such as DNA and RNA extraction, lysate preparation, qPCR, protein quantification, Western blot/WES/JESS, and ELISA is preferred.
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This position reports to the Principal Investigator and the Pre-Clinical Surgeon of the Cardiovascular surgical and interventional research laboratory within the MCRI and requires surgical and anesthesia proficiency in a variety of laboratory animal species and animal models.
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Respond to all client requests, inquiries, and concerns about animal care and facilities operations in timely manner. Manage day-to-day operations of vivarium staff providing animal health checks, husbandry services, and research support services.
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Husbandry, set up mouse breeders and genotyping of transgenic mice to obtain the desired experimental genotypes, in compliance with the approved animal protocols and guidelines of the animal research facility.
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Proficiency in animal handling, dosing, and necropsy is desired. Cellular and molecular biology methodologies, e.g., mammalian cell culture; in vitro screening (e.g., transfection); cell-based assays ; PCR.
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AALAS certification at the Assistant Laboratory Animal Technician level (ALAT) or higher or ability to successfully achieve ALAT certification within one (1) year of hire/promotion. Help train animal care staff in the following: cage changing, microisolator cage changing techniques, autoclave/cagewash operations, and other husbandry duties.
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Veterinary degree (DVM/VMD) from an AVMaccredited school of veterinary medicine or ECFVG (Educational Commission for Foreign Veterinary Graduates) certification with post-graduate work experience at a ACLAM-recognized laboratory animal medicine training program or equivalent.
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As the face of VCA Wakefield Animal Hospital, the client service representative sets the tone for the practice as we build and maintain each customer's trust and confidence in our abilities.
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Will manage adherence to institutional biosafety and animal care and use standards and keep compliance records/logs up to date. Demonstrated animal management experience to include mice husbandry, breeding, colony management, mouse genetics and genotyping procedures.
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Monitors status of institutional review board (IRB) and animal assurance committee (AAC) approval for pending and ongoing studies, and works with investigators to submit new applications, annual reviews, and notification of changes to study design and methods.
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An appointment in OEB provides access to state-of-the-art animal and plant facilities and core facilities for imaging, proteomics, genomics, and bioinformatics as well as access to multiple supporting institutions including the Museum of Comparative Zoology, Harvard University Herbaria, Harvard Forest, and the Arnold Arboretum.
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In addition to basic methods of cell and molecular biology including signal transduction pathways, microRNA and long noncoding RNA, transgenics and gene knock out the Chen Lab has used a number of rodent animal models, in which stem cells, growth factors and advanced matrix scaffolds are applied.
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Additional Skills/Preferences: Mastery of CRISPR-based editing in translational models, including primary cells, pre-clinical disease models, and large animal IND-enabling models. Deep scientific knowledge of lipid nanoparticles and AAV packaging of gene editing components.
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Animal care and husbandry. Animal experimentation and husbandry is routinely performed. Will assist researcher with maintaining breeding units and generating/genotyping experimental animals as well as maintaining online animal database.
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animal job Title: client representative Company: Vca Animal Hospitals in Stoneham, MA
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