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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Extensive hands-on experience with standard immunological techniques including multi-parameter flow cytometry, cell sorting, immunoassays such as ELISA, Cytotoxicity, in vitro co-culture assays (MLR, PBMC cultures, etc), and reporter bioassay development.
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Familiarity with common molecular and cell biology techniques to support ligand characterization (e.g., western blotting, cell culture, assay development, in vitro viability assays.
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An ideal candidate should have a strong background in immortalized (hepatocytes) and primary cell culture (HSC, T cells) and have experience screening in these cell types using various CRISPR delivery systems, including RNPs and LNPs.
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Expression and purification of antibody constructs in mammalian cell culture at micro, medium and large scale as appropriate. We are seeking a highly talented and motivated scientist to join our growing team and establish our in-house Biologics engineering capabilities.
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Cellular and molecular biology methodologies, e.g., mammalian cell culture; in vitro screening (e.g., transfection); cell-based assays ; PCR. Expertise in cellular and molecular biology is essential (e.g., mammalian cell culture; cell-based assays; quantitative real-time PCR.
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Utilize cellular and molecular biology methodologies, such as mammalian cell culture, in vitro screening, cell-based assays, and PCR. PhD in cell or molecular biology or a related field, ideally with 2 or more years post-degree experience preferably in drug discovery and in an industry setting.
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2+ years of hands-on experience with mammalian cell culture and molecular biology. · Perform in vitro assays, including primary human immune cell isolation, culture and differentiation.
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Technical expertise with mammalian cell culture including establishing and maintaining cell lines, transient and stable transfection, and reporter cell lines. Experienced with primary cell culture and characterization, including tissue dissociation and cell isolation of various immune cell types.
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The ideal candidate should possess strong laboratory experience and technical expertise in in vitro cellular immunology (including ELISA, MSD, assay development, tissue culture, flow cytometry, primary immune cell assays, cell isolation) and ex vivo tissue processing and analysis.
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Preferred Experience and Skills: Extensive experience with mammalian cell culture using sterile techniques. On-Board Services is hiring a Molecular/Cell Biology Scientist I based out of Boston, MA.
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We are seeking a talented and motivated individual to join our team as the Principal Scientist Cell Culture. Creative thinker in the field of mammalian cell culture with a track record of innovating in the field of protein production, in particular CHO, upstream development, media optimization and process development, ideally reflected by a strong publication record.
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Significant experience with drug discovery applications of cell culture including immortalized cell lines, primary cell cultures, and multicellular or organoid culture models, preferably models that recapitulate cellular cross-talk in fibroinflammatory or diseased tissues.
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Job Responsibilities: Working with up to 250 L-scale aseptic mammalian suspension (HEK293) cell culture in single-use bioreactors. Experience with Hek293 cell lines, transfection Depth Filtration, Flat sheet TFF Experience in industry leadership Data trending (e.g., metabolites, viable cell density, flux, pressure.
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Strong experience in mammalian cell culture including expertise in aseptic technique, culture optimization, cell counting, cryopreservation, and validation methods. Maintain cell culture, including cryopreservation, validation, and inventory management.
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cell culture jobs Title: scientist in Cambridge, MA
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