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This may include performing tissue collection and processing, flow cytometry or FACS sorting, histology and immunohistochemistry, RNA and DNA extraction, cDNA synthesis and PCR. Conduct analysis of results in consultation with PI. Attend weekly laboratory meetings and maintains laboratory notebooks.
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Preferred capabilities include RNA biochemistry experience, quantitative analysis using techniques like CGE, PAGE, ELISA, HPLC, and experience in analyzing and reporting on analytical data. Operate in-house processes for circular RNA (circRNA) and mRNA production, utilizing techniques such as HPLC or FPLC, chromatogram peak collection and pooling, and reporting in LIMS.
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Performs techniques such as tissue culture, RNA isolation, PCR, immunohistochemistry, electrophoresis, protein purification and others. Under close supervision of Principal Investigator, conducts a variety of routine experiments following established procedures and protocols, which may include performing assays, tissue culture, RNA isolation, electrophoresis, and other laboratory techniques.
Starting at $19.8 - $23.55Full-timeExpandApply NowActive JobUpdated 5 days ago - UpvoteDownvoteShare Job
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Within Ginkgo, the Mammalian Engineering team consists of a set of scientists and engineers with subject matter expertise across cell therapy, gene therapy, rna therapeutics, and biologics. Ginkgo has a number of public and confidential programs in RNA therapeutics, including developing next generation circular RNA and mRNA constructs with Pfizer, and triggering cell-type specific circularization with.
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Experience in most cell biology application areas: Western Blot, Immunohistochemistry, Flow Cytometry, ELISA.Must have experience of selling Life Science reagents to industrial accounts including Pharmaceutical, Therapeutic and Biotechnology companies, and be able to demonstrate sales growth, existing contacts, and insight into the market.
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We are looking for a CTO/CSO, RNA Delivery System (LNP) with a proven track record in biochemical engineering and nanomedicine to lead the development of novel delivery systems for different technology platforms, including mRNA vaccines, therapeutics, siRNA, and in vivo gene editing.
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Ability to develop, evaluate, and implement workflows for technology, optimize and standardized existing techniques and workflows with respect to experimental HT mRNA generation process workflow including DNA & IVT-RNA production, and in vitro/in vivo screening is desired.
ExpandApply NowActive JobUpdated 7 days ago - UpvoteDownvoteShare Job
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Assist with ongoing projects in the laboratory which may involve performing the following activities: cloning, plasmid preps, PCR genotyping analysis, DNA and RNA extraction and purification, preparing samples for sequencing experiments, tissue culture, culturing of fungi and bacteria, microscopy, bioinformatics and/or data analysis.
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Proficiency in mammalian cell culture techniques, flow cytometry, qPCR, DNA and RNA extraction, transfection, ELISA, and western blotting. The current Flagship ecosystem comprises 41 transformative companies, including Axcella Health (NASDAQ: AXLA), Denali Therapeutics (NASDAQ: DNLI), Evelo Biosciences (NASDAQ: EVLO), Foghorn Therapeutics (NASDAQ: FHTX), Indigo Ag, Kaleido Biosciences (NASDAQ: KLDO), Moderna (NASDAQ: MRNA), Rubius Therapeutics (NASDAQ: RUBY), Sana Biotechnology, Seres Therapeutics (NASDAQ: MCRB), and Sigilon Therapeutics (NASDAQ: SGTX.
ExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Under the general supervision of the investigator, performs complex laboratory experiments in the field of cell biology including: cell culture, ELISA, DNA/RNA isolation, PCR, general biochemistry, immunohistochemistry, flow cytometry, and microscopy.
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1 year in molecular biology techniques with hands on expertise in DNA/RNA extraction, PCR, q-PCR, mutagenesis & assembly reactions, SDS-gel page, electrophoresis, cloning replication - pDNA techniques, western, northern, southern blotting, different cell line cultures, affinity chromatography, ELISA, microfluid liquid handling system, and thin layer chromatography.
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Experience in therapeutic drug discovery, gene therapy, or RNA therapeutics. Hands-on experience with cell-based assays and general molecular biology methods including, but not limited to, cell transfection, IVT, RNA extraction, qPCR, ddPCR, FACS, TapeStation, or Fragment Analyzer.
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Routine tests may include virus RNA extraction, PCR, DNA sequencing, real-time PCR, plasma and PBMC isolation from whole blood, PBMC subpopulation isolation, and drug susceptibility assays with HIV-1 and HIV-2.
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Support flow cytometry evaluation of RNA nanoparticles. Formulate and characterize novel formulations for RNA nanoparticles (e.g. small, ultra-small formulation batches, DLS, chromatography.
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You can learn more about us by visiting DescriptionWe are looking for a CTO/CSO, RNA Delivery System (LNP) with a proven track record in biochemical engineering and nanomedicine to lead the development of novel delivery systems for different technology platforms, including mRNA vaccines, therapeutics, siRNA, and in vivo gene editing.
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immunohistochemistry rna jobs in Belmont, MA
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