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Immunohistochemistry and fluorescent microscopy. A Staff Scientist position is available in the laboratory of Drs. Elliot and Glassberg to study translational aspects of lung injury, repair and fibrosis with specific emphasis on the role of vascular dysfunction and aging in the progression of chronic lung diseases.
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Performing immunohistochemistry and confocal/bright field microscopy. Some of the related techniques that may be applied in the research to be conducted will include: microinjection, mass spectrometry, high-throughput screening, mammalian cell culture, cell transfection assays, flow cytometry (FACS), immunoprecipitation/Western blots.
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Experience demonstrating independence in cell culture, immunofluorescence/immunohistochemistry, molecular/cellular biology techniques including DNA and RNA isolation, quantitative real time PCR, protein assays, ELISA, Western blotting, immunoprecipitation, cell proliferation and viability assays and in vivo rodent models.
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Have previous experience with RNA/DNA/protein isolation, RT-PCR, western blot/ELISA, immunohistochemistry/immunofluorescence, and cell culture (sterile technique). Performs/Quantifies RNA/DNA/Protein Isolation, RT-PCR, ELISA/Western Blot, Immunohistochemistry & Immunofluorescence Performs dissociation of esophageal biopsies to single cell suspension.
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Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining Immunohistochemistry. The Northwestern campus sits on the traditional homelands of the people of the Council of Three Fires, the Ojibwe, Potawatomi, and Odawa as well as the Menominee, Miami and Ho-Chunk nations.
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Experimental techniques will include behavioral assessment of mouse models, brain and spinal cord dissection, immunohistochemistry, immunoblotting, EM processing, confocal, TEM imaging, and other biochemical and molecular approaches.
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The Histotechnologist reflects the mission, vision, and values of NM, adheres to the organization’s Code of Ethics and Corporate Compliance Program, and complies with all relevant policies, procedures, guidelines and all other regulatory and accreditation standards.
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Design and perform advanced in vitro and in vivo experiments, including the use of mammalian cell culture, CRISPR gene editing, lentiviral transduction, immunoblotting, immunohistochemistry, and animal procedures.
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The postdoc will work with Dr. Chen to investigate the cytoprotective response pathways in oligodendrocytes in vitro, in a variety of genetic mouse models as well as in mouse models of demyelination. In addition to lab work, the postdoc will teach one or two courses in the Department of Biology.
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Experience with molecular/cellular biology assays (RT-PCR & qPCR, ELISA, Luminex, cell culture, immunohistochemistry, etc.) Experience with molecular/cellular biology assays (RT-PCR & qPCR, ELISA, Luminex, cell culture, immunohistochemistry, etc.
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The Histotechnologist performs histological and microscopy related procedures of a routine and specialized nature including, tissue processing, sectioning, histological staining, and immunohistochemistry.
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With respect to laboratory technical skills, the individual must be proficient in cell/tissue culture, flow cytometry/sorting (FACS), protein isolation and analysis (western blot, ELISA, and immunohistochemistry), RNA isolation and analysis by qRT-PCR, molecular biology (PCR, genotyping, cloning plasmid constructs, DNA isolation and sequencing analysis, CRISPR.
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The position will utilize knowledge of proteomics, molecular biology, neurobiology, cell biology, biochemistry, and animal brain surgery. Animal transcranial perfusion and histology. T cell activation, tail vein injection, student mentoring, and manuscript writing.
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Diversity and Inclusion Statement: Diversity, Equity, and Inclusion As one of the nation’s largest Jesuit, Catholic universities, Loyola University Chicago fosters a transformative cultural experience that honors diversity, equity, and inclusion.
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The postdoctoral fellow will study the response of oligodendrocytes to various stress conditions, including ER stress and inflammation. Loyola University Chicago supports its staff and faculty with a wide array of affordable, comprehensive and competitive benefits centered on health and wellness, financial security, equity, and work-life balance.
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immunohistochemistry job in Chicago, IL
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