NGS Scientist

Responsibilities: Excellent employment opportunity for a Scientist III in the Ridgefield, CT area.Design, develop, and execute functional genomics assays, including CRISPR-based editing (e.g., RNP delivery, Perturb-seq), single-cell sequencing, spatial transcriptomics, and CITE-seq.Provide technical guidance and training to research scientists on FGSL technologies and techniques, including assay execution, troubleshooting, and resolution of technical issues;Documents and maintains detailed laboratory records in accordance with policies;Operates and maintains lab equipment in collaboration with other lab members;Assists acquisition of necessary supplies and equipment;Defines and performs appropriate QC measures;Follows relevant scientific literature to ensure use of optimal methods and understand emerging practices across the field;Demonstrates the ability to interpret the outcome of experiments, propose appropriate follow-up, and may propose new avenues of investigation;Communicates own work effectively orally and in writing; contributes to writing protocols, procedures, and technical reportsAutomate processing and results reporting and delivery;Reports and treats data with a high level of integrity and ethics.Experience: Master's degree from an accredited institution with 4+ years of experience in molecular, cell biology, or related scientific discipline.CRISPR assays - Must have personally designed guides, performed transfections/infections, selected edited cells, and validated knockouts/knockinsNext-generation sequencing (NGS) - Hands-on library preparation experience (not just data analysis)Single cell technologies - Personal experience with sample prep, library generation, and quality controlStandard molecular biology techniques - qPCR, Western blot, cloning, gel electrophoresisCell culture - Proficient in maintaining multiple cell lines, transfections, and sterile techniqueExperience in automation is a plus;Able to perform variety of FGS experimental techniques at the same time;Demonstrated experience working in a multi-disciplinary and collaborative environment;Ability to troubleshoot FGS experiments both individually and as part of a team.Excellent oral and written skills with the ability to communicate in an open, transparent, timely and consistent manner.