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Performing a range of molecular biology techniques, including molecular cloning, DNA and RNA isolation, and protein expression analysis. Research Specialist (Molecular and Cell Biology) - Feng Lab.
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Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.
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Molecular biology and biochemistry: RNA and DNA extractions, cloning, mutagenesis, quantitative PCR, protein immunoprecipitations, Western blotting, and proteomic sample preparation. Stem cell culture: CRISPR editing, stem cell culture, directed differentiation, and fluorescence microscopy of human induced pluripotent stem cells (hiPSCs.
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RTPCR), western blotting, ELISA, Next Generation Sequencing. BS in Molecular Cell Biology or other scientific discipline.
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Strong background in assay development using molecular genomic technologies (both RNA and DNA), including experience in next-generation sequencing, microarray, or other high-throughput platforms.
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Robust experience in RNA sequencing (bulk and single-cell) and proteomics methods. At least 4 years of experience in virology and general molecular biology techniques. Stay updated with the latest molecular biology and multiomics techniques relevant to viral research.
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Strong background in basic research or translational research in oncology, including but not limited to high throughput screening, cell-based assays, protein biochemistry, molecular biology with emphasis on RNA technologies.
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Must have experience with DNA/RNA handling and purification. BS/MS in Biochemistry, Molecular Biology, Biotechnology, or a related discipline. The ideal candidate will work with lead researchers on a variety of molecular biology projects.
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Broad experience and expertise in molecular reagent generation and cell line engineering using gene editing approaches CRISPR as well as development and implementation of novel cellular target engagement activity and PPI assays e.g. immunoassays second messenger generation ion flux reporter protein stability PPI splitNanoLuc split-gal gene activation cellular imaging.
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, Next Generation Sequencing, flow cytometry. Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, Next Generation Sequencing, flow cytometry.
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Basic skill sets required: NGS (library prep, running Illumina Sequencers), Primary T Cell culture, Crispr KO, Transduction, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR. Bachelor’s degree in Molecular Biology, Biology, Biochemistry, Biomedical Engineering or equivalent.
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Existing collaborations within CZ Biohub: Technology Platforms (Bioengineering, Computational Microscopy, Data Science, Genomics & Mass Spectrometry), Infectious Disease , and Quantitative Cell Science.
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Experience with standard laboratory molecular biology software packages, RNA biology, in vivo gene delivery, mouse primary immune cell culture. Currently pursuing a BS degree in Immunology, Molecular Biology, Chemistry or related field.
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Hands-on experience with basic molecular biology techniques, e.g., PCR, DNA/RNA purification, electrophoresis. Our lab seeks a Staff Research Associate (SRA) who will aid scientist(s) on new and ongoing research projects by performing basic molecular biological techniques (including molecular cloning and Western blotting) and cell culture (including iPSC cell culture) in the laboratory.
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Master's or bachelor’s degree in molecular biology, Biophysics, Computational Biology, or related field. You will be involved in developing and deploying high-throughput RNA production and purification platform assist in RNA production to ensure smooth operation.
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rna molecular jobs in San Francisco, CA
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