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Generate antibody fragment libraries for antibody discovery and engineering, perform HT binding screens (ELISA and multi-plex flow cytometry) and sequence analysis to generate hits. Generate antibody fragment libraries for antibody discovery and engineering, perform HT binding screens (ELISA and multi-plex flow cytometry) and sequence analysis to generate hits.
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Reporting to the Cell Therapy lead for our first LINK program, the successful applicant will be part of a team that is characterizing and optimizing one of the company’s core platforms: Logic-gated Intracellular Network (LINK) CAR. Within this role, they will work on understanding core biology of the platform with viral generation, transduction of human T cells, and functional assays involving cytotoxicity, flow cytometry, and long-term persistence.
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Proficiency with stable cell line development, preferably in CHO cells, including transfections, selections, subcloning, flow cytometry, protein measurement by Octet and HPLC. Proficiency with molecular biology techniques including molecular cloning; preparative and analytical DNA/RNA PCR/qPCR/RT-PCR; proficiency with associated software, ex Geneious, Sequencher.
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In vitro work includes a range of cellular assays, cell culture including multiparametric flow cytometry and molecular biology techniques to help interpret in vivo observations. skills: Molecular Biology, Cell Biology, Biology, GLP (Good Laboratory Practice), In Vivo, Flow Cytometry.
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Prior experience with human tissue culture and organoid culture and flow cytometry is a plus. Duration: 12 monthsLocation: South San Francisco, CAAs a Scientific Researcher I, you will be:Job Description:The Scientific Researcher performs laboratory-based activities and processes related to the tracking registration inventory preparation e.g. DNA/RNA extractions from human biospecimens fluid and tissue samples human cell culture organoid culture Serum Plasma and PBMC isolation from blood samples and distribution of biological samples from clinical and non-clinical trials.
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Significant experience in antibody discovery and protein engineering by using single B cell cloning, hybridoma, or phage/yeast display techniques. BS with 10+ years of research experience in antibody or protein engineering.
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Work as part of a team to develop and perform in vitro assays, including but not limited to: modulation of gene expression, cell-based functional assays, flow cytometry, ELISA, qPCR, and high-content cell imaging.
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Expertise in independently leading lab-based research projects involving a wide repertoire of experimental techniques such as conventional/spectral flow cytometry, biochemistry, cell/molecular biology, and immune function assays.
$80.56 an hourExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Following are some expectations for hands on laboratory experience (minimum 2-3 years): 1) DNA/ RNA extraction from human samples (blood, tissue, stool). Truly passionate about the importance of biorepository management, as well as the ability to work in a global, matrix environment.
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Experience with DLS/SLS, CE, ITC, MS, SEC-MALS, ELISA, Flow cytometry, and other analytical methods. Experience with DLS/SLS, CE, ITC, MS, SEC-MALS, ELISA, Flow cytometry, and other analytical methods.
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Experienced in qPCR, Western blot, ICC, IHC, ELISA, FRET, flow cytometry and other standard molecular biology techniques. Experience assaying primary immune cell activation (e.g. flow cytometry.
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Familiarity with in vivo pharmacology, single cell workflows, and spatial omics would be helpful. This is a hands-on, lab-based position in which you will design, perform, and analyze in vitro and ex vivo experiments to validate biological and therapeutic hypotheses derived from clinical biomarker data, real-world data, and other sources.
$86.02 an hourFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Hands-on experience in various phenotypic and functional cell-based assays, such as flow cytometry, HTRF, ELISA, Western/Jess, cell viability assays and other multiplexing technologies. Hands-on experience in various phenotypic and functional cell-based assays, such as flow cytometry, HTRF, ELISA, Western/Jess, cell viability assays and other multiplexing technologies.
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Other responsibilities may include utilizing nonclinical PK and PK/PD data (including use of modeling and simulation techniques) to project optimal clinical dosing regimens, doses based on pharmacological/clinical targets, human starting doses, and biomarker exploratory data (including biochemical and molecular data from quantification of mRNA, multi-panel flow cytometry, and protein expression.
$239,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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This work will require a number of biochemical and biologic techniques including (but not limited to): PCR; DNA and protein gel electrophoresis; Western blotting; standard cell culture; flow cytometry; transfections; DNA/RNA extractions; protein purification; buffer preparation; and microplate assays.
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flow cytometry jobs in San Francisco, CA
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