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Develop and optimize protocols for cell culture, differentiation and tissue engineering. PhD in Cell Biology, Regenerative Medicine, Tissue Engineering, Biomedical Engineering or related field.
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The successful candidate will play a key role in advancing our understanding of plant cell culture processes, with a focus on molecular and cellular biology, genetics, and plant cell culture fermentation.
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Perform production activities including thawing of cell stocks, inoculum train expansions, cell culture maintenance in bioreactors, depth and sterile filtration, and master cell bank and bulk drug substance fills.
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Cell based assays in cell plates, ELISA, Fluorescence detection with microplate reader, Cell culture aseptic techniques, handling, storage and maintenance of primary and cell lines, Cell transfection, lipid-based and/or magnetofection and handling of plasmids, Antibody-based cell separation techniques using magnetic columns.
$68,000 - $81,600 a yearFull-timeExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Candidates must have robust cell culture experience, and an understanding of stem cell biology, the hematopoietic system, and immunology is preferred. B.S. / M.S. with a minimum of 4 years of relevant experience in cell culture techniques.
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Proficiency in standard immunoassay methodologies (high-parameter flow/spectral flow cytometry, ELISA, Luminex, MSD), human T cell culture, and molecular biology techniques (qPCR, ddPCR, NanoString, NGS, scRNSeq, CITE-Seq.
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DESCRIPTIONUnder the supervision of the Principal Investigator (PI), the employee will assist in research on uncovering principles of cellular protein quality control by carrying out various biochemistry, cell biology and molecular biological procedures in mammalian cells and Zebrafish.
$21.39 - $23.06 an hourFull-timeExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Experience in cell culture and cell-based assay development, T-cell biology, and flow cytometry is helpful but not required. Perform hands-on execution of mammalian cell culture experiments in BSL2 environment including DNA plasmid transfection and small-scale virus production, purification and transduction of cell lines.
ExpandApply NowActive JobUpdated 6 days ago - UpvoteDownvoteShare Job
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Experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots, and other standard cell-based or biochemical assays.
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Responsibilities Representative responsibilities will include, but not necessarily be limited to, the following: Collaborate with research, analytical development and process development teams to develop, qualify/validate AAV gene therapy cell-based biological potency assays, protein chemistry assays, and molecular biology assays for process monitoring and drug product release, specifically SDS-PAGE, western blot, droplet digital PCR or qPCR, culture of primary mammalian cells, and cell.
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The Research Assistant I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Molecular biology (cloning, restriction digests) under the supervision of a senior scientist Protein science, including western blot Cell culture, sterile technique, and transfection. Skills such as, histology, cloning, general mammalian cell culture, transfection and familiarity with in vivo rodent models will be considered advantageous.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
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Training in developmental biology, cell culture, microscopy, and fish physiology. Knowledge and experience in molecular biology techniques including genotyping, PCR, DNA/RNA isolation, primer design, qPCR, and in vitro RNA synthesis.
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Experience with mammalian cell culture perfusion bioreactor equipment setup and operation. Experience with pilot-scale (50L to 200L) mammalian cell culture upstream and downstream unit operations.
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cell culture jobs in San Diego, CA
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