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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular. Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis), and molecular.
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Molecular biology (cloning, restriction digests) under the supervision of a senior scientist Protein science, including western blot Cell culture, sterile technique, and transfection.
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Biochemical techniques including quantitative Polymerase Chain Reaction (qPCR)/Digital Droplet PCR (ddPCR), Enzyme-Linked Immunosorbent Assay (ELISA), western blotting, gel-based assays, tissue culture, etc.
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Perform routine bacterial, yeast, and mammalian cell culture with minimal supervision. The Associate will execute reagent production, high-throughput serum bactericidal assays, antibody isolation and characterization, routine cell-culture, and bacterial virulence assays.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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Experience in mammalian cell culture, protein expression platforms (i.e. HEK, CHO, or E. coli), and an understanding of protein purification techniques is preferred. Under guidance and in collaboration with others the candidate will perform research for projects in drug discovery and protein engineering.
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Collaborate with research, analytical development and process development teams to develop, qualify/validate AAV gene therapy cell-based biological potency assays, protein chemistry assays, and molecular biology assays for process monitoring and drug product release, specifically SDS-PAGE, western blot, droplet digital PCR or qPCR, culture of primary mammalian cells, and cell-based potency assays.
$84,000 - $118,667 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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DESCRIPTIONUnder the supervision of the Principal Investigator (PI), the employee will assist in research on uncovering principles of cellular protein quality control by carrying out various biochemistry, cell biology and molecular biological procedures in mammalian cells and Zebrafish.
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Expert in ELISA techniques, flow cytometry, cell culture, immunoassays, antibody purification, and mouse handling, including immunizations, spleen removals, hybridomas, etc. These include intellectual input and decision-making on protein targets to be pursued, development of immunogens and immunization strategies, utilization of hybridoma technology, and antibody validation in vitro and in vivo.
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Skillsets of interest include expertise in tissue culture, biochemical tools (SDS-PAGE, immunoblotting, ELISA, protein assay), and a good working knowledge of molecular techniques including generation of mutant plasmids, transfection with plasmids/siRNA for transient protein expression in cell-based reporter systems as well as use of CRISPR and transcriptomics/proteomics technologies.
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Extensive hands-on experience with Molecular Biology (as PCR, qRT-PCR, ddPCR, DNA/RNA and protein preparation), Cloning, mammalian cell culture and transfection is required. Proficiency with culture of cell lines and primary cells is strongly preferred.
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You are an experienced molecular biologist with a passion for drug discovery and extensive experience with routine molecular and cell biology approaches including plasmid cloning, mammalian tissue culture, cell-based assay development, and CRISPR/RNAi. You have a proven record of supporting projects from the bench through the design and execution of cell-based assays that yield robust, decision-making data packages.
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Education requirements include master’s of science with specific focus in one or more of the following disciplines: cell signaling, cell physiology, protein biochemistry, cancer biology and immunology.
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At least 2 years postgraduate, hands-on experience in mammalian cell culture and protein biochemistry such as SDS-PAGE, ELISA and affinity chromatography are required. The Research Associate will be responsible for conducting hands-on experiments including but not limited to molecular biology, mammalian cell culture, recombinant protein purification and characterization.
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cell culture protein jobs in San Diego, CA
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