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StartYourStory with PPD, part of Thermo Fisher Scientific, where diverse experiences, backgrounds and perspectives are valued. Thermo Fisher Scientific values the health and wellbeing of our employees.
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General techniques: Handling DNA and RNA, protein and nucleic acid electrophoresis; Western blotting; Immunoprecipitation, extract preparation, quantitative enzyme assays (nuclease, polymerase, helicase, topoisomerase or other kinetic analyses), transfection and transformation; light microscopy.
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Significant experience with wet lab techniques for sample processing such as DNA and RNA extraction, DNA sequencing, PCR and microarray. Significant experience with a variety of DNA sequencing technology platforms.
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Specific tasks will involve insect transmission/biological assays, DNA and RNA isolation from plants and insects, cDNA synthesis, primer design; PCR, RT-PCR, routine PCR-, real-time qPCR- and ddPCR- amplification assays; molecular cloning; plasmid purification, colony PCR, Sanger DNA sequencing and sequence analysis; dsRNA synthesis; other contemporary techniques.
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Proficiency in molecular biology techniques such as PCR, DNA sequencing, and plasmid purification. Research Specialist/Post-Doctoral Researcher in Plasmid DNA Engineering for Lipid Nanoparticle-Mediated Drug Delivery.
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Familiarity with commercially available NGS kits for targeted and non-targeted RNA & DNA sequencing. Generate NGS libraries for sequencing of RNA and cell-free DNA. Prior experience working with biofluids, and cell free DNA is a plus.
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Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to DNA and RNA isolation, PCR, gel electrophoresis, DNA assembly, bacterial transformation, mammalian cell culture, etc.
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Are excellent in most of the following: bacterial culturing, PCR, gel electrophoresis, molecular cloning, plate reader assays, cell-free expression systems, protein purification, buffer and media preparation, and DNA purification, sequencing, and analysis.
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Prepare DNA and subsequent libraries for Illumina sequencing. Must have experience with DNA/RNA handling and purification. Existing collaborations within CZ Biohub: Technology Platforms (Bioengineering, Computational Microscopy, Data Science, Genomics & Mass Spectrometry), Infectious Disease , and Quantitative Cell Science.
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Demonstrated experience with protein, DNA, or viral purification by standard chromatography methods as well as fluency with the theoretical concepts underlying protein purification. Demonstrated experience with analytics and characterization of proteins and antibodies (SDS-PAGE, Western Blot, residual Host Cell Proteins, ELISA residual endotoxin, residual DNA, HPLC.
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Optimize pilot plant and pre-clinical production performance to allow for fast, flexible processing of multiple manufacturing processes including plasmid DNA prep, mRNA transcription and purification, and LNP formulation, and oligonucleotide synthesis.
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Isolates, purifies and analyzes, RNA, DNA and protein including hybridization, gel electrophoresis autoradiography, ECL staining, performing PCR reactions, quantitative RT-PCR assay, DNA sequencing and assays for signal transducing enzymes.
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This position will assist graduate and undergraduate students with sample tracking, processing of blood, eggshell, skin, fin clip, and swab samples, and DNA extractions for genotyping and sequencing of fish and wildlife species of conservation concern.
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The ideal candidate for this position will have hands-on laboratory research experience and a working knowledge of molecular biology techniques (e.g. PCR, DNA extraction, sequencing). This will involve managing laboratory experiments including DNA extractions, PCR, and next-gen and Sanger sequencing, preparing and maintaining a large collection of DNA samples, managing the LIMS database that tracks the sample inventory and data, analyzing Sanger and next-gen sequence data, handling equipment installation and repairs, and coordinating research activities with collaborating groups.
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As the Associate Director of Business Development, Synthetic Biology , you will help to promote the awareness of an exciting new DNA synthesis technology to the life science community and lead and oversee activities to grow partnerships in the field of Synthetic Biology.
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dna job Title: technical sales Company: Thermo Fisher Scientific
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