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Overview Axle is seeking a highly ambitious, adaptable, structured, and detail-oriented Flow Cytometry Immunology Scientist to join our vibrant team at the National Institutes of Health (NIH), National Institute of Allergy and Infectious Diseases (NIAID), Vaccine Research Center (VRC), Vaccine Production Program (VPP.
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You have experience with primary human immune cell activation assays using multparameter flow cytometry. You have experience with T cell cytotoxicity assays using endpoint flow cytometry measurements.
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Perform enzymatic assays, ex vivo sample processing, and various molecular biology techniques including flow cytometry, western blot, and ELISA. Utilize plate readers, fluorescence microscopy, and flow cytometry.
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Execution and analysis of multiple flow cytometry-based assays for characterization of cell therapy products and in-vivo tissue samples. The primary responsibilities will involve the isolation of primary lymphocytes for pre-clinical research, CAR-T cell production, and characterization of various products using flow cytometry and other in-vitro assays.
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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Familiarity with IND-enabling studies and preparing materials for the pharmacology section of an IND.Excellent skills with cell culture such as primary cell culture, stable and transient protein expression, fixed and live cell imaging by IHC or immunofluorescence, flow cytometry.
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Extensive hands-on experience with standard immunological techniques including multi-parameter flow cytometry, cell sorting, immunoassays such as ELISA, Cytotoxicity, in vitro co-culture assays (MLR, PBMC cultures, etc), and reporter bioassay development.
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Develop viral vector drug product characterization methodologies including but not limited to particle sizing techniques, flow cytometry, flow imaging and other virus characterization technologies.
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Specific duties include tissue culture, flow cytometry, in vivo murine work, epigenetic profiling, single cell and next generation sequencing, and other basic molecular biology techniques.
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This position additionally requires providing technical support for in vitro and in vivo experiments, encompassing molecular biology, cell culture, and flow cytometry, for the benefit of post-doctoral fellows and graduate students within the laboratory.
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Experience in and/or development of abilities to use equipment items to perform PCR , Western blotting, tissue sections, immunohistochemistry, immunofluorescence, cell culture, flow cytometry, optical coherence tomography, ultracentrifuge, and others.
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Mammalian cell line engineering, mammalian cell culture, molecular biology, flow cytometry, PCR, bench, project management. Flow cytometry, PCR, and other related molecular and cellular biology techniques.
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Experience with confocal microscopy, Incucyte, flow cytometry or other fluorescence-based methodsLilly is an EEO/Affirmative Action Employer and does not discriminate on the basis of age, race, color, religion, gender, sexual orientation, gender identity, gender expression, national origin, protected veteran status, disability or any other legally protected status.
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Experience with serological assays and multicolor flow cytometry is highly preferred. Isolating immune cell populations and performing multicolor flow cytometry. You must have extensive in vivo mouse handling experience including infection/immunization, blood sampling, tissue collection, and immune cell isolation.
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Performs gating, analyses and scientific review of high dimensional flow cytometry data collected from immuno-oncology clinical trials. Must have at least 5 years of experience in analysis of high dimensional (12+ color) flow cytometry data, specifically with T-cell functional subsets (memory, activation, exhaustion) and/or Myeloid cell phenotyping (MDSC, pDC etc.
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vivo flow cytometry associate jobs Company: Adcentrx Therapeutics
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