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Expert in ELISA techniques, flow cytometry, cell culture, immunoassays, antibody purification, and mouse handling, including immunizations, spleen removals, hybridomas, etc. The company is committed to the discovery and development of novel immunotherapeutics (monoclonal antibodies and antibody-drug conjugates) for an array of disease states.
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Desirable but not required: experience in ex-vivo culture of human cells, immune cell separation using magnetic beads, flow cytometry, gene-editing techniques (RNAi, CRISPR), and RT-qPCR.
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Execution and analysis of multiple flow cytometry-based assays for characterization of cell therapy products and in-vivo tissue samples. Proficiency in multi-parameter flow cytometry and FACS data analysis programs.
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Position Summary: The Senior Technician in the Discovery Technologies In vivo pharmacology group will be part of a dynamic group of scientists conducting various research projects to identify large molecule/biologics which can effectively treat various forms of cancer.
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Will gain proficiency in flow cytometry including designing of multicolor panels, titration of antibody staining, and analysis. Experience in fluorescent microscopy, single cell transcriptomics, and flow cytometry preferred.
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Well-versed in cell culture aseptic techniques, multi-color flow cytometry (10+ colors), PCR, MSD/ELISA, or other cellular assays. Experience with spectral flow cytometry.
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Position Description:The Neurophysiology Group within the Research and Development organization is searching for an experienced electrophysiologist to support novel drug discovery research in CNS disorders.
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Perform in vivo studies and ex vivo analysis to facilitate drug discovery and development in various diseases. Preclinical disease model development in the context of drug discovery.
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Experience with confocal microscopy, Incucyte, flow cytometry or other fluorescence-based methodsLilly is an EEO/Affirmative Action Employer and does not discriminate on the basis of age, race, color, religion, gender, sexual orientation, gender identity, gender expression, national origin, protected veteran status, disability or any other legally protected status.
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Demonstrated experience in diverse molecular and biochemical laboratory techniques applied to cell characterization at molecular or genetic levels (immunofluorescence, flow cytometry, qPCR, RNA sequencing)Well organized, self-motivated, team oriented, ability to multitask with attention to detailExcellent communication and writing skillsProficient in computational analysis and coding pipelines (R Studio, Python, etc.
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Experience in operation of multiple flow cytometry platforms (e.g.: BD, Sony, etc.) Support animal study in compliance with Sanofi policies - Basic rodent manipulation and techniques (including but not limited to a range of various blood collection methods, necropsy collections, and tissue processing for flow analysis, etc.
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Responsibilities include cell culture and maintenance of human cell lines, basic molecular biology (PCR, cloning, running DNA and protein gels), flow cytometry, preparation of samples for deep sequencing, confocal microscopy, basic statistical calculations, and other duties as assigned.
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Plays a key role in developing our in vitro and in vivo strategies towards target identification and drug discovery, through to clinical candidate selection. Design and build innovative in vitro & in vivo approaches to screening drug candidates, both internally and through CRO and academic collaborations.
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This work will also entail advanced sequencing techniques to measure transcriptomic and epigenetic changes and, cellular function assays with live microscopy and flow cytometry and biochemical assays.
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The successful candidate will utilize an array of state-of-the-art approaches, including cellular & molecular biology techniques, single-cell RNA sequencing, flow cytometry, confocal microscopy, and AD transgenic animal models, to delineate how senescent cells affect amyloid plaque formation, tau pathologies, neuroinflammation and AD progression.
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