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Experience with standard molecular biology techniques such as PCR, bacterial plasmid production, in vitro transcription. Perform experiments to produce DNA templates for in-vitro transcription.
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Independently conduct molecular biology assays including flow cytometry, biomarker screening, conventional PCR, real time PCR, western blotting, immunohistochemistry and immunofluorescence staining.
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The research specialist will extract RNA, synthesis cDNA, run qRT-PCR and perform cell culture experiments and maintenance. The research specialist will also generate gastrointestinal organoids from tissue and maintain intestinal organoids in vitro.
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In addition to being well versed with tissue engineering techniques, in vitro cell culture and animal models, strong preference will be given to applicants with backgrounds in vascular and/or neurobiology.
$32.43 - $51.81 an hour depends on experienceFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Basic molecular biology and microbiology techniques, such as aseptic technique, bacterial isolation and culture, strain library maintenance, PCR, DNA/RNA/protein extraction, cloning, recombineering, etc.
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Experience in Molecular techniques e.g. PCR (design primers/probe, optimize condition) Valued Complementary Skills:Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Molecular experience in PCR, ddPCR, DNA/RNA extractions/purification is a plus. A basic understanding of Virology and In vitro virus culture experience is a plus. ATCC, a pioneer in biomaterials manufacturing, is seeking a Biologist to work onsite in the BioProduction Virology department in Manassas, VA. Prior experience with mamamalian cell culture and aseptic technique is required.
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Ideal candidates should have experience in standard molecular and cellular biology techniques, including but not limited to mammalian cell culturing, western blotting, cell microscopy, PCR, cell cloning, gene transfections, cell survival, flow cytometry, clonogenic assays, and histochemistry.
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In addition, a candidate must have expertise in at least of three of the following techniques: in vitro osteoblastogenesis and osteoclastogenesis, micro-CT, confocal microscopy, Real-Time-PCR, ELISA, Western-blot, immunofluorescence, brain histology, IVIS imaging, mass-spectrometry.
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Histology (dissection of intact nervous tissue and cryosectioning); troubleshooting IHC with untested antibodies; western blot; cell culture of infected cell lines; Enterovirus D68 handling, expansion and titering; nucleic acid extraction, RT-PCR and qPCR; confocal microscopy; electron microscopy; mouse handling and injection (IM, IP, and IC.
$85,000 - $125,000Full-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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These will include but are not limited to primary tissue processing, flow cytometry and cell sorting, single-cell RNA/DNA/protein/ATAC-sequencing data generation, sequencing library construction, bulk RNA extraction and analysis, human specimens processing and analysis for RT-PCR, cell cultures for in vitro and in vivo experiments, CRISPR screens, western blotting, immunohistochemistry and image analysis.
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Requirements Requirements, Knowledge, Skills, and Abilities:Ph. D. in a scientific discipline (e.g., molecular biology, cell biology, or related field) with 0-2 plus years of hands-on experience with gene editing, site-specific nucleases (especially RNguided nucleases), transposons, or other vectors used in vivo or in vitro, is required.
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Measurement of molecules in vivo: western blot analysis of proteins, northern blot analysis of RNA transcripts, RT-PCR or Real Time Reverse Transcriptase PCR analysis of RNA transcripts.
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Molecular biology and biochemistry experiments: DNA/RNA isolation PCR and qPCR protein isolation western blot analysis immunofluorescence and immunohistochemistry studies. The Ivy Brain Tumor Center is currently recruiting a highly motivated and driven technician to join our In vivo/In vitro team focusing on cutting edge Phase 0/II clinical trials for glioblastoma at BNI. The Ivy Brain Tumor Center is a non-conventional non-profit program offering state-of-the-art clinical trials for patients with the most aggressive form of brain cancer.
$19.73 - $27.13 an hourFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Perform recombinant DNA activities, ranging from cloning (Gibson Assembly, PCR, Vector Digestion) and DNA plasmid purification (small & large-scale) and in vitro transcription to generate assay controls.
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