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Mammalian cell culture, cytotoxicity analysis and multi-color fluorescence flow cytometry. Conduct literature search to design in vitro/in vivo models and conduct thorough research to initiate pipeline development.
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Must possess some but not all of the following laboratory skills: tissue culture, PCR, Western blots, flow cytometry, and immunostaining / immunofluorescence microscopy and analysis, cryostat sectioning.
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Essential laboratory skills including molecular cloning, bioproduction, RNA and protein analysis, tissue culture, fluorescence and confocal microscopy, flow cytometry/sorting and animal handling are highly desired.
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Expertise in the following techniques is mandatory: magnetic cell isolation, cell culture, phenotyping of bone marrow progenitors using multicolor flow cytometry, CFU assays using CD34+ cells, ELISA, MSD, Western blot for signaling pathway proteins, basic molecular biology including isolation of nucleic acid and real-time PCR.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Experience in flow cytometry, ELISA with preferred experience in MSD and/or Luminex. Working with multiparameter flow cytometry, including cell surface staining, intracellular staining, data acquisition and analysis.
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The ideal candidate has direct experience with mammalian tissue culture, protein purification, proficiency in standard chromatography techniques, protein characterization (e.g., MS, SEC), and binding assays (e.g., ELISA and flow cytometry.
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Proficient in basic cell characterization techniques, including flow cytometry, ddPCR, qPCR, ELISA. Experience in CRO and/or scientific team management is an asset. Key Responsibilities: Lead preclinical studies that provide scientific rationale for advancement to human trials, including safety, efficacy, and mechanisms of action studies using clinically relevant in vitro and in vivo models.
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Two or more years experience in conducting immunological assays including immunophenotyping/flow cytometry on a diverse assortment of primary immune cells, FACS, Luminex cytokine profiling and analysis, magnetic bead enrichments for population analysis, ELISpot, and ELISAs.
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Direct experience performing in vitro functional assays is preferred, including cytotoxicity, proliferation, multi-color flow cytometry and cytokine release. Perform phenotypic analysis of engineered CAR T cells using multicolor flow cytometry.
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Develop and implement functional assays, including IncuCyte, eSight, RTCA, ELISA, MSD, and multicolor flow cytometry. Lead the design, development, and optimization of T-cell candidates using in vitro and in vivo assays.
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Hands-on experience in Cell Culture, ELISA/MSD, Flow Cytometry, PCR (NGS is a plus) Under supervision, execute and then lead development activities for cell-based methods, flow cytometry, qPCR / ddPCR and ELISA.
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Relevant experience with T cells and other immune cells in vitro and in vivo, immunological techniques, and multicolor flow cytometry are highly desirable. CRISPR, RNA/DNA analyses (RNseq), immunohistochemistry, immunoprecipitation, immunoblotting, and general lab organization/safety.
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, Next Generation Sequencing, flow cytometry. Cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay.
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Our laboratory is recruiting a postdoctoral fellow to use unbiased approaches (RNSeq, scRNSeq, spatial profiling, and flow cytometry) to identify novel molecular and cellular events in solid cancers with different treatments.
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