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Viral DNA, RNA and protein extraction, purification, and analysis, including cloning, amplification and DNA sequencing. Sample preparation for DNA sequencing. Analysis of viral growth by microscopy and enzyme assays.
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The candidate will provide leadership in biomarker assay development, implementation and sample analysis. Broad knowledge and in-depth experience in biomarker assay method development, qualification/validation and sample analysis in support of preclinical or clinical studies.
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Research duties may include the handling of research animals (including rats, rabbits, sheep, and/or horses) including gait analysis procedures, collection of animal tissues, sample processing, tissue culture according to defined protocols, PCR, RNA analysis, antibody staining for flow cytometric analysis, and histology/immunohistochemistry.
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Run sensitive, reliable and efficient LC-MS/MS bioanalytical assays for TK/PK assessment Perform high-volume sample analysis and/or data analysis, data QC, and data interpretation Maintain schedule of projects and timelines Communicate project timelines, status, and data to Principle Investigator effectively addressing any issues that may arise Simultaneously handle method development routine sample analysis projects as required.
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The postdoc will provide appropriate spatial transcriptomic sample setup and preparation support and execution for investigators who are seeking answers to biologically important questions and will integrate with the Tulane Center for Aging Genomics and Bioinformatics cores to provide a seamless pipeline for sequencing and bioinformatic analysis.
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The successful candidate will provide scientific and technical overview for tissue-based methods that span sample processing, assay development, whole slide scanning, image QC, analysis, and data reporting.
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Automated, unbiased sample selection and scalable study design will immediately facilitate deep spatial investigations in landmark MD Anderson programs, including APOLLO and Patient MOSAIC, to direct novel data analysis, enable translational studies, and generate clinical impact.
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The individual will be expected to have good organizational skills, attention to detail, and solid experience in wet lab work; enzyme incubations, in vivo sample handling, and/or organic extractions preferred.
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Experience with multi-parameter flow cytometry panel design, sample acquisition, and data analysis. The candidate will be responsible for receipt, shipping, and storage of human specimens, database management, ordering and maintain laboratory supplies, developing new immunology assay protocols, conducting immunology experiments, data analysis, quality assurance/quality control, and participating in lab meetings and journal clubs.
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Perform assays to include viral and bacterial stock characterization, plating assays, ELISA, RT-PCR, microarray, cytokine analysis, and applicable in vitro sample analysis assays as required by approved in vivo and in vitro protocols.
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Must possess knowledge of laboratory analytical theories, techniques and instrumentation employed in the quantitation/analysis of drugs and metabolites (e.g., immunoassay, UV/Vis Fluorescence plate readers, HPLC, mass spectrometry, automated sample processing equipment, etc.
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The ideal candidate should possess a strong background in chemistry, have a thorough understanding of cGMP, GLP, GDP guidelines, and be proficient in utilizing analytical instruments such as FTIR, HPLC, and ICP. Additionally, the Lab Chemist will collaborate closely with third-party laboratories, manage sample submissions, and generate Certificates of Analysis (COAs) to meet industry standards and regulations.
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Once the sample is preserved in the 'live' state, it is then possible to study the ultrastructure of these samples using not only basic Electron Microscopy imaging techniques, but also state-of the-art techniques such as: 1) 3-D electron tomography (cryo and plastic), 2) Single Particle Analysis (SPA), and 3) CLEM.
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Performs physician-ordered arterial blood sample collection and performs subsequent analysis on an arterial blood gas analyzer for oxygen, carbon dioxide, pH, and oxygen saturation levels.
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The Research Associate will support the validation of Client therapeutic candidates by performing hands-on, bench-level research involving both cell culture and tissue-based experiments, as well as sample processing and analyses from in vivo animal studies.
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