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Overseeing and developing assays, from RNA and DNA including: sequencing (NGS, pyrosequencing, Sanger) and DNA fragment analysis. The candidate will join a team of molecular scientists and directors in our oncology-focused molecular genetics laboratory in Phoenix, Arizona.
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Provides consultation for specialized biomarker analyses including genomic analysis of RNA expression data and analysis of other biomarker data (single cell RNA, NGS, protein, imaging) in conjunction with pre-clinical and clinical data.
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Possible CRISPR guide RNA design and molecular cloning. Basic handling of DNA and RNA including sample isolation, PCR, and preparation of samples for sequencing. We are a recently established translational leukemia research laboratory that uses a variety of biochemical, molecular, and cell biology approaches to identify new therapeutic targets, develop preclinical models of disease, and test novel therapeutics both in vitro and in vivo using mouse models.
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Senior Scientist – RNA Chemistry SUMMARY: Intellia Therapeutics is seeking an experienced Senior Scientist to join the RNA Chemistry Team, which is responsible for the production of synthetic oligonucleotides.
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Experience with cellular and molecular biology techniques (including molecular cloning, RNA extraction, PCR, qPCR, western blotting, and microscopy). Associate Research Scientist - RNA Biology , Aging & Cancer.
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This is an exciting opportunity for BS, MS, and PhD level individuals with immunology, cell biology, and/or molecular biology experience to learn and execute key techniques including culturing primary human cells and cell lines, CRISPR editing, biochemical assay development, ELISA and other cytokine-based assays, RNA isolation and quantitative PCR, and flow cytometry/FACS and microscopy.
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Develop RNA purification, characterization, and analytical methods to generate therapeutic grade material for in vitro and in vivo studies. Strong RNA biology knowledge including genetic element (UTRs, codon, structure, IRES) optimization for enhanced protein translation.
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LHRI has defined novel mechanisms of drug resistance and viral fitness in people living with HIV/AIDS (PLWHA) and novel Innate immune responses against RNA or DNA virus infection. RNA handling, including in vitro RNA transcription and RNA transfection.
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The incubent will work alongside subject matter experts in the fields of cell biology and biomedical engineering, and perform functional and targeted assays to describe the neurobiology of the model systems, such as RNA isolation/sequencing, ELISA, and other in vitro assays.
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You will carry out DNA/RNA NGS library preparation, sequencing, and analysis to produce high quality, consistent data to drive product improvements. Minimum of 2 – 5 years related experience in handling DNA, RNA, including extraction, purification, and quality assessment techniques.
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General duties will include breeding, weaning and genotyping genetically altered mice, assisting in collection, processing and cataloging tissue samples from mice, culture of primary cells, RNA and protein extraction, performing various biologic analyses (gel electrophoresis, Western blotting, ELISA, immunohistochemistry and immunocytochemistry, qRT-PCR, etc.
$31,561 - $58,393 a yearFull-timeExpandApply NowActive JobUpdated 14 days ago - UpvoteDownvoteShare Job
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Perform preclinical studies involving RNA and DNA delivery using Piezo’s platform via. Experience with handling nucleic acids (such as RNA) is preferred while drug delivery of RNbased vaccines and therapeutics is a significant bonus.
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Performing molecular biology assays (PCR, RNA extraction, library preparation). We are looking for an organized, motivated research specialist who can contribute to our work on RNA virus evolution and transmission.
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Familiarity with DNA, RNA, enzyme, antibody, and antigen chemistry is highly desirable. Our team engages in a wide range of activities, from cutting-edge organic synthesis to innovative formulations chemistry, developing novel coatings, and supporting an array of other programs.
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3 years of experience with molecular biology techniques and in vitro assays, including DNA/RNA extraction, qPCR, immunofluorescence, and Western blotting. 4 years of experience with molecular biology techniques and in vitro assays, including DNA/RNA extraction, qPCR, immunofluorescence, and Western blotting.
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