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Next-generation sequencing, digital PCR/qPCR, and/or Mass Spectrometry experience. Demonstrated success working in PCR or NGS assay design and manufacturing. Leading our national digital PCR and NGS testing department to ensure SQDC goals are met.
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Current projects in the laboratory require in vitro cell culture, work with primary cells and organotypic 3D skin equivalents, fluorescent microscopy, histology, flow cytometry, and molecular biology (real time RT-PCR, Western blots, transfections, siRNA etc.
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Primary responsibilities include fabrication of DNA constructs using a combination of PCR, restriction enzyme digestion, ligation, transformation, DNA extraction, and DNA sequencing. Tissue cultureMolecular cloning and DNA assembly with E. coliPCRDNA electrophoresisDNA purification and cleanup (i.e. PCR cleanups, gel purification)Restriction digestion, ligation (or other assembly techniques)DNA transformationPlating microbial transformation on appropriate conditionsPlasmid DNA purification (i.e. mini prepping)DNA sequence sample preparation (sanger) and analysisExcellent sterile techniqueExcellent pipette and liquid-handling techniqueExcellent record-keeping.
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Design and develop a structured process for improvement and coordinate product/process changes (ECR / PCR process) Prior experience with CNC programming (HAAS, Mazak) Experience using Solidworks, Creo, or Pro-E.
$38 - $46 an hourFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Assay performance: Perform ELISAs, RT-PCR, quantitative PCR and protein characterization (Western blots), endotoxin assays, biocompatibility testing (complement and cytokine ELISA and Luminex arrays), and other specialized blood assays.
$55,000 - $70,000 a yearFull-timeExpandUpdated 1 month ago - UpvoteDownvoteShare Job
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Assisting in training and managing undergraduate students, shipping and receiving animals and reagents, performing PCR, RT PCR, genotyping, DNA/RNA isolation and other miscellaneous experiments.
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Develop other molecular biology-based assays using techniques such as cloning, DNA/RNA/protein extraction and RT-PCR. Highly experienced in protein assay development such as western blot/JESS, flow cytometry, ELISA, AlphaLisa, and immunoprecipitation.
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Experience in molecular biology techniques, such as PCR and NGS library synthesis, is required. Perform Molecular Biology techniques such as PCR and NGS library generation. The One Functional Genomics team within the GRC provides expertise in genome editing and CRISPR technology to discover and validate novel drug targets for the therapeutic pipelines in Immunology, Oncology and Neuroscience.
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Minimum of 2 years of industry experience in a molecular biology atmosphere involving RT-PCR assays. Experience with nucleic acid sample preparation methods, rapid PCR technologies and various clinical matrices.
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Expertise in Flow Cytometry, FACS cell sorters, FlowJo, PCR etc. Skilled in Assay Development, Cell isolation, Cell engineering, Gene editing (CRISPR, Lentiviral, Retroviral) This post is to recruit another scientist in immuno-oncology to join our interdisciplinary team.
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Perform recombinant DNA activities, ranging from cloning (Gibson Assembly, PCR, Vector Digestion) and DNA plasmid purification (small & large-scale) and in vitro transcription to generate assay controls.
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RTPCR), western blotting, ELISA, Next Generation Sequencing. Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RTPCR), western blotting, ELISA, Next Generation Sequencing.
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Candidates are expected to be familiar with the following techniques: cell culture, transfections, cloning, DNA and RNA isolation, immunoblotting, PCR, real-time PCR, MTT assays, cytofluorimetry, CRISPR gene editing.
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The Department's 18 divisions include Academic General Pediatrics, Child Development & Community Health; Allergy, Immunology, and Rheumatology; Cardiology; Dermatology; Dysmorphology; Emergency Medicine; Endocrinology; Gastroenterology; Genetics; Genome Information Sciences; Hospital Medicine; Infectious Diseases; Neonatology; Nephrology; Pharmacology & Drug Discovery; Rehabilitation Medicine; and Respiratory Medicine.
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Strong understanding and demonstrated practical experience with a range of molecular biology technologies including cloning and other DNA/RNA manipulations, PCR, qPCR, and western blotting. This position will be part of a highly matrixed, cross-functional discovery team with opportunities to interact across protein engineering, plant pathology and analytical chemistry functional teams.
Full-timeExpandUpdated 16 days ago
pcr job
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