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Biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR , Flow Cytometry ) transductions , RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and.
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The incumbent will be responsible for drug screening, cell-based assay development, and biomarker discovery. Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular.
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Hands on experience in working with CRISPR, siRNA and shRNA is a plus. Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral. This Associate Research Scientist will join the Translational Development Group in San Diego.
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A strong background in standard molecular biology (such as PCR, qRT-PCR, ddPCR, DNA/RNA extraction and molecular cloning) is required. Previous experience using methods to investigate/query transposon or viral vector integration sites, such as LM-PCR or LAM-PCR, is preferred.
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Duties include overseeing the daily running of the lab, purchasing and organizing supplies and reagents, keeping lab internal databases, assisting in training and managing undergraduate students, shipping and receiving animals and reagents, performing PCR, RT PCR, genotyping, DNA/RNA isolation and other miscellaneous experiments.
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The Department’s 18 divisions include Academic General Pediatrics, Child Development & Community Health; Allergy, Immunology, and Rheumatology; Cardiology; Dermatology; Dysmorphology; Emergency Medicine; Endocrinology; Gastroenterology; Genetics; Genome Information Sciences; Hospital Medicine; Infectious Diseases; Neonatology; Nephrology; Pharmacology & Drug Discovery; Rehabilitation Medicine; and Respiratory Medicine.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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Work at the bench to develop and execute established and novel molecular and cell-based assays for drug screening as well as understanding molecular pathways (Proliferation, Apoptosis, Flow Cytometry etc.
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Performance of molecular testing (PCR, Microarray, Sanger/NGS sequencing) a plus. Active California Department of Public Health Clinical Laboratory Scientist (CLS) license or Clinical Genomics Molecular Biology Scientist (CGMBS) license (no exceptions.
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Biochemical techniques including quantitative Polymerase Chain Reaction (qPCR)/Digital Droplet PCR (ddPCR), Enzyme-Linked Immunosorbent Assay (ELISA), western blotting, gel-based assays, etc. High-Performance Liquid Chromatography (HPLC), Liquid Chromatography Mass Spectrometry (LCMS), Fragment Analyzer, Capillary Electrophoresis.
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Job Description: Job Title: Associate Research Scientist. drug screening as well as understanding molecular pathways (Proliferation, Apoptosis, Flow. ELISA. Some training may be provided. Work at the bench to develop and execute established and Client molecular and cell-based assays for.
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Lab techniques include a combination of in vivo mouse work and bench work ( in situ hybridization and RNAscope, qRT PCR, immunohistochemistry, hormone ELISAs, brain histology and neuroanatomy, microscopy, viral vectors, RNAseq, etc.
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You will routinely design and perform genome editing using CRISPR tools and other molecular biology techniques such as PCR, Gibson assembly, DNA transformation, and DNA sequencing analysis. You will routinely design and perform genome editing using CRISPR tools and other molecular biology techniques such as PCR, Gibson assembly, DNA transformation, and DNA sequencing analysis.
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Requirements Experience with human cell culture, protein assays (plate based ELISAs, immunostaining-based assays, fluorescent microscopy), quantitative PCR, RNA and protein isolation and quantification methods required.
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Strong understanding and demonstrated practical experience with a range of molecular biology technologies including cloning and other DNA/RNA manipulations, PCR, qPCR, and western blotting. This position will be part of a highly matrixed, cross-functional discovery team with opportunities to interact across protein engineering, plant pathology and analytical chemistry functional teams.
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pcr job in San Diego, CA
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