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Experience and knowledge on multi-color flow cytometry, including staining, analysis and fluorescence-activated cell sorting. Experience and knowledge on multi-color flow cytometry, including staining, analysis and fluorescence-activated cell sorting.
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Under the direction of the Registered Dietitian, the Degreed Nutritionist determines eligibility, provides nutrition education classes and counseling services for program participants. The SDSU Research Foundation Women, Infants, and Children (WIC) Program is the largest WIC agency in San Diego County, with approximately 80 employees providing a variety of nutrition services to over 26,000 participants monthly.
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Delivers person centered care as an integral member of the Adult Health Services multidisciplinary team at WIHD. The Nutritionist provides nutrition management Nutrition management of chronic disease among individuals with genetic and metabolic disorders to include Prader-Willi syndrome, Cystic Fibrosis, Down syndrome, Phenylketonuria (PKU), Fragile X syndrome, Rett syndrome and Williams syndrome.
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Must be board eligible for Registered Dietitian (RD) or Registered Dietitian Nutritionist (RDN) by the Commission on Dietetic Registration (CDR); required within 3 months of hire. Must be board eligible for Registered Dietitian (RD) or Registered Dietitian Nutritionist (RDN) by the Commission on Dietetic Registration (CDR); required within 3 months of hire.
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Within 6 months (180 days) of hire: Washington Nutritionist Certification. Upon hire: National CDR Registered Dietitian - Commission on Dietetic Registration. Dietitian - PacMed Beacon Hill - Nutrition Services.
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Conduct assays and experiments using flow cytometry, PCR, cell-based assays/bioassay development, and ELISA techniques. Design and execute cell-based assays (AAV transduction, transfection, flow cytometry, luciferase assays, and immunofluorescence.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Other responsibilities may include utilizing nonclinical PK and PK/PD data (including use of modeling and simulation techniques) to project optimal clinical dosing regimens, doses based on pharmacological/clinical targets, human starting doses, and biomarker exploratory data (including biochemical and molecular data from quantification of mRNA, multi-panel flow cytometry, and protein expression.
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Basic knowledge of mouse neuroanatomy, genetics, flow cytometry, RNA sequencing, electrophysiology and/or cell biology. Basic knowledge of mouse neuroanatomy, genetics, flow cytometry, RNA sequencing, electrophysiology and/or cell biology.
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Contribute to the project development as well as perform cellular immunological techniques, including multi-color flow cytometry, ELISA, MSD and in vitro immune cell assays. Contribute to the project development as well as perform cellular immunological techniques, including multi-color flow cytometry, ELISA, MSD and in vitro immune cell assays.
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Experienced in designing, developing, performing, and analyzing in vivo/ex vivo studies, use of contemporary cellular and molecular, immunological techniques, RNA analysis, flow cytometry, protein analysis by Western/MSD/ELISA, and associated data analysis.
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Lead in the pre-sales and sales activities to properly promote the Company's Flow Cytometry portfolio of products to ensure financial targets are achieved. Bachelor's degree or 5-7 years of experience in flow cytometry, immunology, cell biology or related pathology discipline required.
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Experience with translational in vitro and ex vivo functional assays using, primary cells, human and rodent tissue samples and human matrices with readouts such as multi-parametric flow cytometry, multiplex ELISA, and application of these techniques to pharmacological studies.
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Screen libraries via common affinity characterization methods such as ELISA, MSD, BLI, and FLOW cytometry. Proficiency with immunoassays; such as ELISA, MSD, and bio-layer interferometry (BLI), and flow cytometry is desired.
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Prior experience with one or more analytical techniques used for characterization and/or quantitation of protein products, such as: chromatography (SEC, IEC, RPLC), electrophoresis (cIEF, CGE), immunoassay (ELISA), bioassay/cell-based (ADCC, flow cytometry), or device functionality (BL/GF) methods.
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