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Execute molecular and cell-based methodologies including flow cytometry, immunohistochemistry, cytotoxicity assays, reporter assays, ELISA, western blotting, WES and in vitro PD assays. Execute molecular and cell-based methodologies including flow cytometry, immunohistochemistry, cytotoxicity assays, reporter assays, ELISA, western blotting, WES and in vitro PD assays.
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Significant experience with cell and gene therapy test methodologies (qPCR/ddPCR, Flow Cytometry, ELISA, and cell-based assays). Significant experience with cell and gene therapy test methodologies (qPCR/ddPCR, Flow Cytometry, ELISA, and cell-based assays.
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Work collaboratively with research, process, and analytics teams to develop and characterize analytical assays in various platforms including but not limited to flow cytometry, ELISA/MSD, qPCR, and ddPCR.
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Assist in interpretation and troubleshooting of analytical assays in various platforms including, flow cytometry, ELISA/MSD, qPCR and ddPCR. Collect and interpret data, prepare experimental reports, and present findings to the IO Process team.
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The Research Associate II, Analytical Testing, will be responsible for performing various analytical assays, including but not limited to ddPCR, RT-qPCR, ELISA, enzymatic assays, and cell-based assays, to characterize several gene therapy vectors.
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Demonstrated proficiency in common analytical techniques, such as PCR, SDS-PAGE, Western blotting and ELISA. This includes but is not limited to ddPCR, qPCR, RT-qPCR, ELISA, enzymatic assays, and cell-based assays.
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Deep technical understanding of analytical method development for key assays (Flow Cytometry, CCV, ddPCR, qPCR, ELISA, HPLC, cell-based potency) and associated requirements (validation, stability programs, comparability assessments, etc.
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Other analytical techniques such as ELISA, HPLC, ELISPOT assay. BS or MS in Biology, Molecular Biology, Biochemistry, or a related discipline with 2+ years laboratory research experience. Other analytical techniques such as ELISA, HPLC, ELISPOT assay.
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Experience with cellular assays (examples: MSD/Luminex/ELISA, multicolor flow cytometry) and/or molecular assays (examples: qPCR/PCR/ddPCR) Experience with cellular assays (examples: MSD/Luminex/ELISA, multicolor flow cytometry) and/or molecular assays (examples: qPCR/PCR/ddPCR.
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Develop and perform cellular and molecular assay techniques (qPCR, dPCR, ELISA, flow cytometry) Familiarity with diverse analytical techniques such as Western Blot, ELISA, qPCR. Develop and perform cellular and molecular assay techniques (qPCR, dPCR, ELISA, flow cytometry.
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Proficient in molecular and cell based analytical methods including cell culture, In vitro cell based assays, ddPCR, ELISA, and flow cytometry. Proficient in molecular and cell based analytical methods including cell culture, In vitro cell based assays, ddPCR, ELISA, and flow cytometry.
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Hands-on experience with common nucleic acid- and protein-based in-process analytic assays (e.g., PCR, RTPCR, ELISA, SDS-PAGE, Western blot) Plasmid and viral vector cloning, qPCR/ ddPCR, wet lab next-generation sequencing (NGS.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Proficiency in a broad range of analytical techniques used in cell and gene therapies (qPCR/dPCR, Flow Cytometry, ELISA, HPLC) Proficiency in a broad range of analytical techniques used in cell and gene therapies (qPCR/dPCR, Flow Cytometry, ELISA, HPLC.
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At least five (5) years of experience with at least 1+ years in a supervisory position of a QC laboratory in the Biotechnology, Biopharmaceutical or Pharmaceutical industry working with GMP. Specific experience should include but not be limited to: ELISA testing, Protein assays, gel-electrophoresis, particulate visual inspection, raw materials testing, and general laboratory supervision and maintenance (inventory control, equipment qualification, personnel training.
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