Thermo Fisher Scientific

Task 2: Support lead scientist to reverse-engineer pre-existing high-producing CHO clones into PoC SSI candidates. Plasmid and viral vector cloning, qPCR/ ddPCR, wet lab next-generation sequencing (NGS). Bachelor's degree in molecular biology, cell biology, virology, or other vaccinology-related discipline or equivalent and relevant formal academic / vocational qualification. Hands-on experience with aseptic small scale (plate- to shake flask-based) mammalian cell culture (thawing, expanding, freezing), transfection techniques, and clonal isolation. Hands-on experience with common nucleic acid- and protein-based in-process analytic assays (e.g., PCR, RTPCR, ELISA, SDS-PAGE, Western blot)

senior scientist

Job Description Thermo Fisher's clinical research business is a leading global contract research organization and world leader in serving science. We are passionate, deliberate, and driven by our mission - to enable our customers to make the world healthier, safer, and cleaner. Within our Analytical Services team, we have a functional service provider solution, which is a unique partnership that allows our customers to leverage the experience of our staff, while allowing you, the employee, to gain direct experience working onsite at a pharmaceutical/ biopharmaceutical company, all while maintaining full-time benefits. Job Responsibilities: Task 1: Support lead scientist to deliver top candidates of site-specific integration (SSI) platform in the Client's Vx CHO cells through a genome-wide screening approach Deliverables: a. Design and synthesis (outsourcing) of necessary constructs b. Production of stable pools and clones c. Screening for clones with target profile (stability and productivity) Task 2: Support lead scientist to reverse-engineer pre-existing high-producing CHO clones into PoC SSI candidates Deliverables: a. Design and synthesis (outsourcing) of necessary constructs b. Production of engineered CHO clones c. Screening for clones with targeted insertion profile Preferred Qualifications: •Excellent communication skills and ability to effectively collaborate within multiple project workstreams •Critical thinking skills and ability to efficiently manage changes in priorities and timelines •Attention to detail and meticulous execution of project-related tasks •Plasmid and viral vector cloning, qPCR/ ddPCR, wet lab next-generation sequencing (NGS) •Experience with FACS analyser/single cell sorter •Experience with CRISPR or other genomic editing tools •Applies detailed fundamental knowledge of own specialty area or scientific discipline into solving process development problems - Experience with Mammalian Cell Culture •Identifies and prioritizes solutions using knowledge gained through formal education, experience, and sound judgment •Fully understands the rationale and hypotheses for experimental designs Education and Experience: Bachelor's degree in molecular biology, cell biology, virology, or other vaccinology-related discipline or equivalent and relevant formal academic / vocational qualification Previous experience that provides the knowledge, skills, and abilities to perform the job (comparable to 4+ years). In some cases, an equivalency, consisting of a combination of appropriate education, training and/or directly related experience, will be considered sufficient for an individual to meet the requirements of the role. Knowledge, Skills and Abilities: <ul><li>Proficient in Microsoft Excel and Word</li><li>Relevant research and development experience in an industrial, academic, or government laboratory setting</li><li>Hands-on experience with aseptic small scale (plate- to shake flask-based) mammalian cell culture (thawing, expanding, freezing), transfection techniques, and clonal isolation</li><li>Hands-on experience with common nucleic acid- and protein-based in-process analytic assays (e.g., PCR, RTPCR, ELISA, SDS-PAGE, Western blot)</li><li>Experience with mammalian cell culture is preferred</li><li>Fluency in English</li><li>Proven ability to interpret data by performing trend analysis</li><li>Proven ability in technical writing skills</li><li>Ability to independently optimize analytical methods</li><li>Proven problem solving and troubleshooting abilities</li><li>Good written and oral communication skills</li><li>Time management and project management skills</li><li>Ability to work in a collaborative work environment with a team</li></ul> Working Environment: <ul><li>PPD values the health and well being of our employees. We support and encourage individuals to create a healthy and balanced environment where they can thrive. Below is listed the working environment/requirements for this role: </li><li>Able to communicate, receive, and understand information and ideas with diverse groups of people in a comprehensible and reasonable manner. </li><li>Able to work upright and stationary and/or standing for typical working hours. </li><li>Able to lift and move objects up to 25 pounds </li><li>Able to work in non-traditional work environments. </li><li>Able to use and learn standard office equipment and technology with proficiency.</li><li>May have exposure to potentially hazardous elements, including infectious agents, typically found in healthcare or laboratory environments. </li><li>Able to perform successfully under pressure while prioritizing and handling multiple projects or activities.</li></ul>

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