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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Perform a variety of biochemical assays including PBMC isolation, flow cytometry, ELISA, western blots. Perform a variety of biochemical assays including PBMC isolation, flow cytometry, ELISA, western blots.
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Testing performed: HLA Flow PRA screen; Luminex Single Antigen (LSA) HLA Class I & II; Flow cross-matching; ELISA; cell isolation and preservation from peripheral blood and donor tissue specimen; DNA extraction, purification, and quantitation; various molecular typing techniques to include Next-generation sequencing (NGS), RT-PCR, and low to intermediate resolution SSO/SSP typing.
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Experience in preclinical malaria mouse challenge models, mosquito dissection, IVIS imaging, flow cytometry, fluorescence microscopy, antibody/protein purification, ELISA, ELISPOT.
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Experience in most cell biology application areas: Western Blot, Immunohistochemistry, Flow Cytometry, ELISA.Must be prepared to travel extensively up to 70% of the time within the territory (some overnight travel required)Independent, driven and able to work on own initiative.
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Broad technical expertise/knowledge including with flow cytometry, ELISA, immunohistochemistry, qRT-PCR, NGS and RNA Seq. Broad technical expertise/knowledge including with flow cytometry, ELISA, immunohistochemistry, qRT-PCR, NGS and RNA Seq.
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Assist in the execution of cell-based assays, including cell viability assays, transfection/transduction assays, flow cytometry, and ELISA. Conduct molecular biology techniques such as DNA/RNA isolation, PCR, qPCR, cloning, and sequencing to support vector design and characterization.
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Considerable expertise relevant to QC analytical methods used for cell therapy manufacturing including qPCR/dPCR, ELISA, flow cytometry or cell-based assays in a GMP environment. Considerable expertise relevant to QC analytical methods used for cell therapy manufacturing including qPCR/dPCR, ELISA, flow cytometry or cell-based assays in a GMP environment.
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Experience in cellular immunology including experience with multiparameter flow cytometry, cell isolation and culture, immunohistochemical staining of tissues, ELISA assays, and ELISpot assays.
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Discovery Biology, biochemical/biophysical assays, cellular assays, transient transfection, viral transduction, CRISPR, Cas9, GraphPad Prism, Genedata Screener, R, FRET, ELISA, MSD, flow cytometry.
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In vitro cellular assays, mammalian cell culture, Lentiviral Transduction, 2D/3D cell culture, SDS-PAGE/Western blot, qPCR, CRISPR, Luciferase, CellTiter-Glo, TR-FRET/BRET, MSD, GraphPad Prism, Genedata Screener, R, immunofluorescence, luminescence, cell viability, FRET, ELISA, MSD, flow cytometry.
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Strong experience with binding assays (e.g. ELISA, flow cytometry) In this role, you will be responsible for the design and execution of cell- and protein- based functional characterization assays for lead antibody identification/optimization and collaborating to generate stage-gate appropriate in vitro / cellular / in vivo data packages supporting PoC. The ideal candidate should have strong experience working with antibodies, significant experience in immunoassays (such as ELISAs) and familiarity with automation technologies is highly valued.
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Analysis of flow cytometry results will be done using FlowJo or Cyflogic programs and involves analysis of fluorescence readouts, live/dead cells discrimination and multi color compensation.
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The Research Associate II is responsible for performing flow cytometry and other immunological assays in support of scientific investigations. Perform multicolor flow cytometric assays using conventional and spectral flow cytometry.
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Aiding in the execution of ELISA, imaging, flow cytometry experiments. Aiding in the execution of ELISA, imaging, flow cytometry experiments. This role requires experience with mammalian cell culture techniques and understanding mechanism of action (MOA) assays.
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