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Capable of performing antibody assays (ELISA or Luminex, including isotyping and avidity assays). Experience with techniques relevant to immunological response characterization (flow cytometry, cell stimulations for cytokine secretion, etc.
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Familiarity with the following cellular and biochemical readouts, including immunofluorescence, luminescence, cell viability, FRET, ELISA, MSD, flow cytometry, is a bonusAbout ActalentActalent is a global leader in engineering and sciences services and talent solutions.
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Demonstrated ability with cell-based assays and molecular biology techniques including, but not limited to, immunoassays (ELISA, Western Blots), PCR, qPCR, and flow cytometry. + Hands on experience with culturing primary cells and cell lines, and experience processing tissues or samples for cell isolation or other downstream applications.
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Provide mechanistic data from preclinical mouse studies and/or in vitro cell-based assays, utilizing flow cytometry, molecular biology, or otheranalyticaltechniques through thecharacterization of immune parameters such as cell expansion, distribution and phenotype, cytokine profile, from ex vivo samples (blood, plasma, serum, tumor, tissues.
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Experience in assay development, mammalian cell culture, ELISA, Western blotting, qPCR, and experience in standard molecular biology techniques such as cloning, protein expression and purification is preferred.
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Proficiency in mammalian cell culture techniques, flow cytometry, qPCR, DNA and RNA extraction, transfection, ELISA, and western blotting. MS/MSc in Biology, Cell Biology, molecular biology, and/or related fields and 2+ year industry experience.
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Position Responsibilities: Perform cGMP analytical testing (bioassay, flow cytometry, qPCR, ELISA, and UPLC) Review and evaluate raw data from a peer or technical review level of all analytical methods, as well as sample authorization.
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Sterile tissue culture techniques, cell-based imaging and assays (immunohistochemistry, fluorescence microscopy, confocal microscopy, ELISA) and mesenchymal stem cell biology experience are also preferred.
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Perform assays on samples collected from mouse and tissue culture experiments, including but not limited to, RT-qPCR, ELISA (single and multiplex), immunohistochemistry, Seahorse assays, and flow cytometry.
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In-depth experience with developing ligand binding assays (e.g., ELISA, MSD, AlphaLISA, Simple Plex, Luminex). The Scientist will independently develop and execute plate-based assays in support of process development as well as for characterization and release of autologous and allogenic cell therapies which are undergoing clinical trials, including CAR T cell products.
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Experience with ELISA, qPCR, ddPCR, SDS-PAGE, and other non-cell based methods. Experience in basic molecular biology (i.e. RNA,/DNA/plasmid extraction, qPCR, ddPCR, etc.) Transfer of test protocols from Analytical development lab (or clients) to cGMP compliant QC laboratory.
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Highly familiar with standard analytics equipment and procedures including, but not limited to Flow cytometry, ELISA, DNA/RNA analysis, cell-based and screening assays, protein analysis, sterility, mycoplasma, endotoxin, cell-counting, qPCR, ddPCR, IHC/pathology.
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Familiarity with cellular and biochemical readouts, including immunofluorescence, luminescence, cell viability, FRET, ELISA, MSD, and flow cytometry. Discovery Biology, biochemical/biophysical assays, cellular assays, transient transfection, viral transduction, CRISPR, Cas9, GraphPad Prism, Genedata Screener, R, FRET, ELISA, MSD, flow cytometry.
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Research Associate, Protein Science, protein expression, purification, microbial cell culturing, molecular cloning, biochemical synthesis, FPLC, SDS-PAGE, ELISA, colorimetric assays, Microsoft Office.
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Experience with mammalian cell culture, and in standard molecular biology techniques such as cloning, DNA purification, and protein expression is preferred. Experience with mammalian cell culture, and in standard molecular biology techniques such as cloning, DNA purification, and protein expression is preferred.
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