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Proficiency in cell culture, cytotoxicity, immunogenicity, neutralizing antibody assays, biomarker identification, ADME/DMPK studies, and potency evaluations. Familiarity with assay technologies such as flow cytometry, qPCR, ddPCR, ELISA, and MSD.
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Thorough general knowledge of bioassay test methods (e.g. Elisa, Flow cytometry, qPCR, cell culture) Support Bioanalytical laboratory by execution of in-process and release testing on batches including, but not limited to, flow cytometry, IFNg potency, qPCR, cell count and viability, sterility, and endotoxin.
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Experience with mammalian cell culture, ELISA assays, formulations and micro pipetting. Perform mammalian cell culture, transient transfections, protein expressions, protein purifications, and micro pipetting in a BSL2 laboratory setting.
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Cell culture and bioassay/potency assay experience is plus. Demonstrated experience with a range of protein/glycoprotein analytical techniques including pH, Conductivity, Osmolality, Protein concentration determination by A280, BCA, Bradford assay, ELISA, HPLC, enzymatic, and electrophoretic (SDS-PAGE, Western blots, IEF and CE) procedures is desirable.
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A- Mammalian or insect cell culture technique. Mammalian or insect cell culture techniques. b-Development of ELISA assays. Development of ELISA assays. Bacterial culture, benchtop (agar, broth up to 1L) to smaller fermenter (up to 10 Liters.
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Hands on experience with cell culture (primary immune cells and established cell lines) and the utilization of cell-based assays to characterize RNbased products (e.g., Multicolor flow cytometry, ELISA/MSD/ELLA, Taqman RT-qPCR, Luminescence, and single cell RNAseq.
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Hands on knowledge of fermentation, cell culture, chromatography, and characterization techniques. Characterize biologics utilizing analytical methods such as ELISA, High Performance Liquid Chromatography, Sodium Dodecyl (lauryl) Sulfate - Polyacrylamide Gel Electrophoresis, and similar.
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Hands-on skills in basic biochemical and immunological methods including cell culture, western blotting, immunoprecipitation, multi-color flow cytometry, ELISA/multiplex assays, ELISPOT, and cell killing assays.
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Generates plasmids for producing lentiviruses to manipulate gene expression in cell culture (shRNA, CRISPR). Duties will include cell culture, cloning, virus generation and cell transformation, as well as some animal work.
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Experience in assay development, mammalian cell culture, ELISA, Western blotting, qPCR, and experience in standard molecular biology techniques such as cloning, protein expression and purification is preferred.
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Activities are mostly focused on wet-lab experiments with human biological specimens, cells, and post-mortem tissues, including cell culture, isolation of nucleic acids, PCR, epigenetics, ELISA, microarrays, microscopy, and molecular biology techniques.
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Cell culture, immunohistochemistry, molecular assays, Western blotting, protein purification, ELISA, etc) and synthesizes and interprets results. cell culture, immunohistochemistry, molecular assays, Western blotting, protein purification, ELISA, etc) and synthesizes and interprets results.
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Preferred qualifications: Familiar with techniques such as multi-color flow cytometry and/or mass cytometry, sterile technique for cell culture with BSL-2+ practices, ELISA, qRT-PCR, and sterile technique for bacterial culture.
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Knowledge of general lab assays is required (e.g., Cell culture, ELISA, Flow Cytometry, Molecular Biology), but experience is preferred. In addition, this individual will conduct some routine characterization testing, using various laboratory methods (e.g., ELISA, Molecular Biology.
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A solid working knowledge of molecular & cell biology techniques including but not limited to nucleic acid/protein handling, molecular cloning, gene expression/silencing/editing (CRISPR), cell culture.
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