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Techniques include: high parameter flow cytometry (up to 27 colors), in vivo mouse work, fluorescent microscopy, confocal microscopy, standard tissue culture, standard mouse handling techniques, ELISA, ELISpot, 10x genomics, single cell PCR.
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Skillsets of interest include expertise in tissue culture, biochemical tools (SDS-PAGE, immunoblotting, ELISA, protein assay), and a good working knowledge of molecular techniques including generation of mutant plasmids, transfection with plasmids/siRNA for transient protein expression in cell-based reporter systems as well as use of CRISPR and transcriptomics/proteomics technologies.
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Perform various laboratory techniques and procedures, such as PCR, ELISA, cell culture, microscopy, and DNA extraction. Proficiency in a wide range of laboratory techniques and procedures, with hands-on experience in PCR, ELISA, cell culture, and molecular biology techniques.
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Daily tasks will involve cell culture (primary cultures and cell lines), Western blot, immunofluorescence, and ELISA. Additional duties may include equipment maintenance, preparation of stock laboratory reagents, and maintaining an organized laboratory notebook.
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Specific Experience: Individual most possess comprehensive knowledge and experience in molecular biology techniques including but not limited to cell culture, RT-PCR, western blotting, nucleic acid isolation and purification, basic histology, and ELISA design and performance.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Technical responsibilities include but are not limited to: maintenance of DNA strain collection, mammalian cell culture, expression and purification of recombinant proteins, antibody purification, IgG quantification by ELISA, SPR, and western blot.
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Support execution of laboratory procedures such as ELISA, immunohistochemistry, cell culture, and microscopy. Our commitment to you is a culture of care and support in which you feel engaged, empowered, and passionate about the work that you perform while working collaboratively and with empathy towards others.
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In addition to being well versed with tissue engineering techniques, in vitro cell culture and animal models, strong preference will be given to applicants with backgrounds in vascular and/or neurobiology.
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Tissue Culture work: maintenance of in vitro and ex vivo (primary) cell culture. Operation of additional immune and in vitro assays (e.g., ELISpot, ELISA, CH50) Experience with sterile tissue culture.
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Prepare reagents, solutions, and culture media for experiments and assays. Harba Solutions is a leading scientific services firm operating across a range of scientific industries including pharmaceuticals, biotechnology, medical devices, contract research organizations, and agricultural sciences.
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Feeds cultures to maintain viability, passages cell lines and splits culture populations, prepares culture populations for long-term storage in liquid nitrogen, administers experimental treatments to culture environments, and measures responsive elements, as appropriate.
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Must have experience with diagnostic, clinical and immunological including but not limited to ELISA, multiplex immune assays such as Luminex, Quanterix or MSD, qRT-PCR, cell culture, piccolo tests, etc.
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Have hands-on experience in the development, optimization, and execution of cellular assays, applying multiple techniques including primary cell isolation, tissue culture, ELISA, flow cytometry, and Western and multiplexing technologies.
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Cell culture and aseptic technique, cell-based potency assays, ELISA, FRET, qPCR, or plate-based biochemical assays (impurity, HCP), or flow cytometry. Cell culture and aseptic technique, cell-based potency assays, ELISA, FRET, qPCR, or plate-based biochemical assays (impurity, HCP), or flow cytometry.
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