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Experience in Molecular techniques e.g. PCR (design primers/probe, optimize condition) Valued Complementary Skills:Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
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Performs routine analytical testing including complex methods such as ELISA, bioassays, and Cell Culture for in process, product release, and stability samples on a daily basis. Recent and relevant experience performing ELISA, Bioassays or Potency testing.
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Utilizes professional knowledge of: theories, principles, methods, and techniques of immunology, cell biology, and microbial pathogenesis, such as ELISA, ASC assays, cellular proliferation assays, western blotting, cell culture, small animal handling (such as mice or guinea pigs), and aseptic technique.
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Strong knowledge and hands-on experience in cell biology and biochemical assays such as cell culture, ELISA and Western-blot. The individual will have experience in developing and optimizing broad and versatile biochemical and molecular biology assays (such as ELISA, WES, PCR.
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Perform immunoassays (MSD, ELISA, etc) for analysis of soluble proteins in cell supernatants/murine plasma. Expertise in cell culture and aseptic technique, involving both immortalized cell lines and primary human/murine cells.
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Nice-to-haves include prior experience with automation, antibody engineering, BLI, and mammalian cell culture. Experience with ELISA and MSD for running high-throughput multiplexed immunoassays.
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Proficiency with tissue culture techniques, including cell culture (cell line and primary cells) and cell transfection using lipofection and lipid nanoparticles. · Proficiency with standard cell biology techniques, including but not limited to fluorescence microscopy, flow cytometry, and ELISA.
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She/he will perform advance cellular and molecular biology techniques under the supervision of the lab manager and PI. She/he will work independently performing several techniques including cell culture, cell transfection, lentiviral particles preparation, flow cytometry, ELISA, molecular biology techniques and animal models.
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Solid understanding and functional knowledge with hands-on experience with at least one of the following: Multicolor Flow Cytometry, Cell-based ELISA, cell culture with cell enumeration (NC-200.
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The ideal candidate will possess a robust technical proficiency encompassing PCR, flow cytometry, cell culturing, and ELISA methodologies. Experience in aseptic processing and mammalian cell culture production.
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Experience in the following techniques: Mammalian Cell Culture, Plaque Titer, ELISA, qPCR, Southern Blot and Western Blot. Experience in the following techniques: Mammalian Cell Culture, Plaque Titer, ELISA, qPCR, Southern Blot and Western Blot.
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Cellular in vitro functional assays, e.g. secondary messenger accumulation assays, reporter gene assays, FRET / BRET assays, real-time kinetic assays, receptor binding assays, and/or ELISA. Prior experience with sterile cell culture techniques and cell engineering.
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Techniques include: high parameter flow cytometry (up to 27 colors), in vivo mouse work, fluorescent microscopy, confocal microscopy, standard tissue culture, standard mouse handling techniques, ELISA, ELISpot, 10x genomics, single cell PCR.
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Skillsets of interest include expertise in tissue culture, biochemical tools (SDS-PAGE, immunoblotting, ELISA, protein assay), and a good working knowledge of molecular techniques including generation of mutant plasmids, transfection with plasmids/siRNA for transient protein expression in cell-based reporter systems as well as use of CRISPR and transcriptomics/proteomics technologies.
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Perform various laboratory techniques and procedures, such as PCR, ELISA, cell culture, microscopy, and DNA extraction. Proficiency in a wide range of laboratory techniques and procedures, with hands-on experience in PCR, ELISA, cell culture, and molecular biology techniques.
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