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Biochemical techniques, mammalian cell culture, assessment of genome editing technologies in bacteria and mammalian cells, DNA sequencing (Sanger and NGS), designing and executing experiments, analyzing and visualizing data, assisting with routine laboratory tasks (organizing, ordering, stocking, etc.
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Overseeing and developing assays, from RNA and DNA including: sequencing (NGS, pyrosequencing, Sanger) and DNA fragment analysis. Evaluating genomic profiles of tumor and blood specimens (NGS based.
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The Staff Scientist, Bioinformatics is responsible for collecting product requirements and designing and developing NGS analysis pipelines to meet those requirements. As a Staff Scientist, Bioinformatics, you will be part of a growing team of bioinformaticians primarily focused on single-cell and immune profiling NGS applications.
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NGS, RNA and DNA analysis. Experience with Cell culture methods (T cells, CAR-T or TCR-T preferred), CRISPR based gene knockout, quantitative PCR and functional characterization of knockout T cells (Cytotoxicity assay using Incucyte or xCelligence, and MLR assay.
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Previous experience with PCR, ddPCR, qRT-PCR, qPCR, NGS. Conduct quantitation and QC of DNA and RNA samples. Execute studies by doing cell culture, qRT-PCR, nucleic acid extraction, and high-throughput sequencing.
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Analysis may include immunological procedures including Enzyme Linked immunoassay (ELISA), Western blot, Flow Cytometry and RNA/DNA sequencing assays. Perform research in next generation sequencing (NGS) including Illumina (iSeq, MiniSeq, MiSeq, NextSeq 2000 and NovaSeq 6000), and Oxford Nanopore Technologies (MinION and GridION.
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Integrated DNA Technologies (IDT) is the leading manufacturer of custom oligonucleotides and proprietary technologies for genomics applications. Experience handling enzymes, DNA, and RNA.
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Experience with techniques such as PCR, molecular cloning, electrophoresis, densitometry, RNA/DNA quality/quantification/fragment analysis, next-generation sequencing (NGS) and enzyme characterization would be an advantage.
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Work Performed Testing performed: HLA Flow PRA screen; Luminex Single Antigen (LSA) HLA Class I & II; Flow cross-matching; ELISA; cell isolation and preservation from peripheral blood and donor tissue specimen; DNA extraction, purification, and quantitation; various molecular typing techniques to include Next-generation sequencing (NGS), RT-PCR, and low to intermediate resolution SSO/SSP typing.
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Operate and analyze data collected on Western Blot equipment such as Protein Simple WES, DNA fragmentation using Covaris systems, Real-Time PCR Detection System such as BioRad CFX, and quantification and QC of RNA and DNA using NanoDrop 8000, Qubit 3.0 Fluorometer, and Agilent 2200 and 4200 TapeStation systems.
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Preparation of DNA, RNA, and ATAC-seq libraries from bulk and/or single cells for NGS sequencing. We are seeking a talented and highly motivated scientist with an interest in advancing Next Generation Sequencing (NGS) technologies for genomics research, to join our Bioinformatics and Sequencing group in Santa Monica.
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Understanding the user of bioinformatics tools including common OMICs Data [dna, rna, methylation, 16S, metabolomics] Understanding the user of bioinformatics tools including common NGS tools [BWA, samtools, GATK.
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Optimize DNA sequencing with NGS platforms. We are seeking an enthusiastic scientist to join our NGS Product Development team as a Scientist II or staff scientist, depending on the qualifications.
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Tasks will include applications of recombinant DNA technology, PCR, DNA sequencing and analysis via traditional and NGS approaches, RNA in vitro synthesis and analysis, and human tissue culture cell work.
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Prepare DNA and subsequent libraries for Illumina sequencing. Must have experience with DNA/RNA handling and purification. NGS library preparation, sequencing, analysis is highly desired.
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