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Experience in confocal microscopy, advanced molecular biology, genomic data analysis, and mammalian cell culture are highly desirable. Experiments will involve cutting-edge cell and molecular biology techniques including differentiation of iPSCs and iPSC-derived RPE cells, spatial transcriptomes/ multiomics analysis, andadvanced microscopy imaging tools.
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Perform production activities including thawing of cell stocks, inoculum train expansions, cell culture maintenance in bioreactors, depth and sterile filtration, and master cell bank and bulk drug substance fills.
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Proven mammalian cell culture and bioassay experience with recombinant and primary cells. Maintains order in cell culture and analytical laboratory, and adheres to cleanroom guidelines and aseptic technique, according to protocol.
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Additionally, the specialist will assist with next-generation sequencing, cell culture, and the development and implementation of high-quality polychromatic flow cytometry and T cell-based assays.
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In this role, you will perform cell culture work, immunohistochemistry/immunofluorescence, DNA/RNA/protein assays, research data procurement, and data analysis. Knowledge of cell culture (including primary cells and cell lines) and virus production (Lentivirus) and transduction.
$28.04 an hourFull-timeExpandUpdated Today - UpvoteDownvoteShare Job
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The Associate Research Specialist will perform molecular biology techniques (cloning, plasmid generation, DNA and RNA isolations), protein work including Western blots, generate lentivirus, culture neurosphere cell lines, and perform functional genomics experiments in these cell lines.
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Valued Complementary SkillsFlow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
$55,000 a yearExpandUpdated Today - UpvoteDownvoteShare Job
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Support early & late-stage CMC development activities including cell line development (CLD), cell culture development (CCD), & purification development for product quantitation, product quality & process impurities for biologic molecules.
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Basic skillsets required: mammalian cell culture, ELISA, Flow Cytometry, DNA/RNA extraction, and PCR is major plus for this role. Mammalian cell culture: feeding and maintaining the health of primary T cells, prepare cell culture media experience is plus.
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Essential Functions Assist in performing in-vitro experiment such as cell culture (maintenance and test on cancer cells or organoids), molecular biology (DNA/RNA extraction, PCR, cloning, biochemical assays and, western blot) and in-vivo experiments such as mouse breeding and genotyping, tumor engraftment and growth.
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Perform routine tasks such as media/buffer preparation, thawing of cryopreserved cells, cell culture passaging, aseptic flask sampling, bioreactor sampling, and cell banking to support ongoing projects.
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As a Research Technologist, your role responsibilities include: Sets up and performs routine and established lab procedures in support of lab goals, grant commitments, i.e. assays, cell/tissue culture, animal studies, etc.
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Ligland binding technology platforms (ELISA & MSD & Luminex)- Aseptic Cell Culture Techniques- Knowledge in basic Protein chaacterization (SDS Page Gel & Western Blots)- Expertise using SoftMac PRO and GraphPad Prism Software.
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The Candidate Bachelors or Masters degree with 4-6 years minimum of industry experience OR PhD with 1-2 years minimum of industry experience within a pharmaceutical or biotech environment Prior experience in Process Development for Cell and Gene Therapy is required Experience with mammalian tissue culture including both adherent and suspension cells.
$85,000 - $110,000 a yearFull-timeExpandUpdated Today - UpvoteDownvoteShare Job
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The applicant will train students and junior members of the laboratory in equipment operation and laboratory techniques, including flow cytometry, mass cytometry, PCR, ELISA, biochemistry techniques and cell culture among others.
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