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Develop and optimize protocols for cell culture, differentiation and tissue engineering. PhD in Cell Biology, Regenerative Medicine, Tissue Engineering, Biomedical Engineering or related field.
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Working under the general supervision of a Senior Technical Research Assistant, Manager or Principal Investigator and following established policies and procedures, conducts a variety of specialized experimental procedures in human iPS cell culture and differentiation, Human fibroblast culture, fluorescence microscopy, FACS analysis, cell biology assays and molecular biology techniques.
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The Senior Research Associate, Bioprocess Development will be responsible for running cell and tissue culture processes as a member of the Tissue Process Development sub-team to support the development and scale-up of novel platforms for meat cultivation.
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PCR machines, electrophoresis equipment, stereomicroscopes, epifluorescence microscopes, laser scanning confocal microscopes, centrifuges, tissue culture cabinets, spectophotometers and plate readers, and autoclaves.
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Previous tissue culture/cell culture experience and flow cytometry experience is strongly preferred. As a lab, we perform analyses that include flow cytometry/fluorescence activated cell sorting, enzyme-linked immunosorbent assays, other bead-based and colorimetric assays, and RNsequencing.
Full-timeExpandApply NowActive JobUpdated 3 days ago - UpvoteDownvoteShare Job
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Execute the necessary molecular biology, tissue culture, biological assays, and related data processing and analysis. High level of proficiency in mammalian cell culture (transfection, gDNA harvest, cell line generation), flow cytometry, and high throughput workflows.
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Experience in recombinant cell line development, primary cell line isolation, tissue digestion. Proven mammalian cell culture and bioassay experience with recombinant and primary cells.
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The role of the Specialist will be to: Willingness and ability to learn a spectrum of new methods and skills Mammalian tissue culture, CRISPR engineering and generation of stable cell lines, in vitro and cellular assays Molecular biology techniques including basic cloning, plasmid design and preparation, DNA extraction, immunofluorescence, western blotting, flow cytometry, etc.
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Required technical skills include: RNA and DNA extraction, tissue culture, and flow cytometry techniques. Areas of expertise include genomics, epigenetics, drug resistance, stem cell biology, drug discovery, diagnostics and biomarker development, and pharmacology.
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Genetics and Cytometry - Nucleic acid extraction, cell culture and harvesting, renal transplant kit oversight and patient testing scheduling and monitoring. Histology and Cytology - Cytology specimen processing, slide preparation and staining, Histology specimen processing, including bone marrow grossing and knowledge of processing for each tissue type.
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The Research Staff Assistant assists a senior student with the development of yeast therapeutics: Engineering yeast for high titer production of biologics using high/throughput methods and characterization of proteins by ELISA and Western Blot. Purifying and characterizing proteins by tagging technologies, FPLC, size exclusion and ion exchange chromatography, mass spec, in vitro tissue culture and biochemical assays.
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Research techniques involve gel electrophoresis, preparation of DNA and RNA, plasmid cloning, preparation of microbial cells, preparation of recombinant media and appropriate growth conditions, tissue culture maintenance and transfection as well as imaging using state of the art microscopy techniques and perform image analysis and data management.
$21.39 - $23.06 an hourFull-timeExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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This position requires experience in intestinal organoid growth, cell culture, and mouse models of gastrointestinal diseases, preferably alcohol or radiation-induced tissue injury.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linked immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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University of Texas MD Anderson Cancer Center: Research Assistant I - Genitourinary Medical Oncology
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Performs cell culture of tumor cell lines and 3D tissue-engineered cultures based on polymeric scaffolds for organotypic tumor growth in vitro. The primary purpose of the Research Assistant I position is to perform basic research functions in the field of bone metastasis, with a strong focus in in vivo imaging, particularly macroscopic bioluminescence imaging and multiphoton/confocal microscopy, and tissue engineering.
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