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Cell culture work, and data entry into a REDCap-based biorepository database. DNA/RNA extraction, PBMC purification, etc.) CHOP’s Commitment to Diversity, Equity, and InclusionCHOP is committed to building an inclusive culture where employees feel a sense of belonging, connection, and community within their workplace.
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In particular, we are interested in candidates with extensive experience in areas including protein biochemistry and purification, enzyme assay development, mammalian cell culture and genetic (e.g., CRISPR-based) screening, and general molecular and cellular biology skills.
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Seeking a highly motivated scientist to support the molecular biology & protein production workflows: DNA construct design/assembly, sample preparation for sequencing, DNA production & quantitation, mammalian cell culture & transfections, protein purification & quantitation, and automated liquid handling.
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1-5 years experience in viral vector/cell therapy (or biopharmaceutical) based GMP manufacturing operations including direct experience in cell culture, cell selection, recovery, purification, and/or aseptic fill/finish.
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Solid working knowledge of various manufacturing platforms (Microbial fermentation process, IB recovery and refolding, CHO cell culture and purification, Pegylation, PEGylated protein purification etc.
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Cellular in vitro functional assays, e.g. secondary messenger accumulation assays, reporter gene assays, FRET / BRET assays, real-time kinetic assays, receptor binding assays, and/or ELISA. Prior experience with sterile cell culture techniques and cell engineering.
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BioServices encompasses Media Prep, Buffer Prep, and general equipment cleaning and preparation supporting both Cell Culture and Purification. Duties are listed below and are specific to the assigned area (Cell Culture, Purification, or BioServices.
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Provide support of lab activities related to the structural biology of viruses and vaccine particles and vaccine development, such as but not limited to: prokaryotic and eukaryotic cell culture techniques for the production and purification of influenza viruses and the production of recombinant protein antigens and antibodies by gradient centrifugation and column chromatography.
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The candidate will have opportunities to work in cell culture development and purification development to become a well-rounded process development scientist with experience in upstream and downstream.
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Expertise in tech transfer, GMPs, facility fit, cell biology, cell culture technology, protein purification, protein chemistry and/or bioengineering. Experience must include four (4) years relevant industry experience in cell biology, cell culture technology, protein purification, protein chemistry and/or bioengineering.
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Proficiency in RNA and DNA purification, qPCR, cloning and mammalian cell culture. We have a caring, supportive culture where everyone feels included, respected, and has equitable opportunities to do their best work.
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Expert in ELISA techniques, flow cytometry, cell culture, immunoassays, antibody purification, and mouse handling, including immunizations, spleen removals, hybridomas, etc. More specifically, select and design immunogens during the monoclonal antibody discovery phase, set and implement immunization strategies, and generate, screen, and evaluate hybridomas and B cell candidates.
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The ideal candidate will have hands-on experience working with mammalian cell culture, protein expression, purification and analytical QC using diverse biochemical methods such as ELISA, SDS-PAGE, Western blotting.
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Experience in cell culture and/or protein purification. skills: Molecular Biology, Cell Biology, Biology, Chemistry, Chromatography, Protein Purification, Cell Cultures, Manufacturing Operations.
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Keywords: Manufacturing Supervisor, Manufacturing manager, bioprocess, manufacturing, batch record, cell culture, upstream, downstream, all stream, fill finish, central services, GMP, aseptic, harvest prep, harvest, sanitation, purification, QC, quality control, equipment, calibration, buffers, solutes, media prep, mixing chemicals, pH, bioprocessing operations, lentiviral.
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cell culture purification jobs
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