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In ideal case, employee will have experience with techniques such as cell culture, DNA and RNA purification and analysis, real-time PCR (qPCR), gel electrophoresis, liquid chromatography, protein purification, immunocytochemistry, flow cytometry, proteomics and mass spec analysis, and bioinformatics skills and a record of published manuscripts in peer-reviewed journals.
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The RA I will be able to perform routine cellular, micro-and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Provide assistance in the execution of experiments and analytical procedures including: molecular biology, DNA cloning, cell culture, protein production, ELISA, Biolayer interferometry and HPLC.
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Experience in mammalian cell culture, protein expression platforms (i.e. HEK, CHO, or E. coli), and an understanding of protein purification techniques is preferred. Skills in molecular biology including DNA isolation and purification, construct design, and cloning are required.
$41,746 - $75,000 a yearFull-timeExpandApply NowActive JobUpdated 24 days ago - UpvoteDownvoteShare Job
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Research techniques involve gel electrophoresis, preparation of DNA and RNA, plasmid cloning, preparation of microbial cells, preparation of recombinant media and appropriate growth conditions, tissue culture maintenance and transfection as well as imaging using state of the art microscopy techniques and perform image analysis and data management.
$21.39 - $23.06 an hourFull-timeExpandApply NowActive JobUpdated 4 days ago - UpvoteDownvoteShare Job
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Theoretical knowledge of or experience with biological and immunological techniques such as cell culture, in vitro experimentation and immunodetection techniques such as ELISA. Theoretical knowledge of or experience with mammalian cellular biology techniques and mammalian cell culture.
$25.75 - $30.72 an hourPart-timeExpandApply NowActive JobUpdated 3 days ago - UpvoteDownvoteShare Job
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The incumbent will perform tibial fractures of mice with various genotypes, evaluate the fractured bones at different times following fracture using methods including histomorphometry, immunohistochemistry, quantitative computed tomography, lineage tracing, cell culture, and genome analysis analyzing the effect of abaloparatide on these processes.
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Mammalian cell culture experience (culturing, transfecting and transducing cells, and DNA/RNA purification from cells). Support cell culture activities and experiments in multiple cell lines, at small and large scales.
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The successful candidate must have previous experience and be proficient in mouse husbandry, PCR-based genotyping, cell culture, flow cytometry, as well as DNA, RNA, and protein isolation and detection techniques.
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Specific Responsibilities:Molecular Biology:DNA, RNA, Protein isolation, purification and expression Western blottingPCR, RT-PCRPlasmid preparationCloning, mapping and sequencing of DNA Recombinant virus production and purificationCellular Biology:Cell culture, cell isolation Functional studies TransfectionImmunologyAntibody production and purificationAdministrationMaintains detailed records of results which may include collecting, extracting & entering data.
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The Associate Research Specialist will perform molecular biology techniques (cloning, plasmid generation, DNA and RNA isolations), protein work including Western blots, generate lentivirus, culture neurosphere cell lines, and perform functional genomics experiments in these cell lines.
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Required technical skills include: RNA and DNA extraction, tissue culture, and flow cytometry techniques. Areas of expertise include genomics, epigenetics, drug resistance, stem cell biology, drug discovery, diagnostics and biomarker development, and pharmacology.
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Responsible for general lab maintenance and organization Preferred Qualifications: Prior experience in isolating DNA, RNA, and mitochondria, cellular metabolism and metabolomics studies, western blot methods, PCR. Extensive hands-on experience on operating and maintaining cell culture and cell-based cytotoxicity assays.
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Proficiency in mammalian cell culture techniques, including cell line maintenance, transfection/transduction, and assay development. Maintain and propagate mammalian cell lines, including primary cells and established cell lines, through aseptic techniques and proper cell culture practices.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
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